Non-Cochrane · DTA · PM41524839
MicroRNAs as diagnostic biomarkers in periodontitis: a systematic review and meta-analysis.
Memon Maaz Anwer, Shahidan Wan Nazatul Shima, Mizusawa Noriko, et al. · Odontology · 2026 · PubMed · doi:10.1007/s10266-025-01299-8
Findings
82 findings
| Population | ||||
|---|---|---|---|---|
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-146 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Positive predictive value (PPV) Disease prevalence 50% | PPV 0.865 [0.499, 0.500] GRADE not reported 3 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-146 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Negative predictive value (NPV) Disease prevalence 20% | NPV 0.963 [0.800, 0.800] GRADE not reported 3 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-146 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled specificity qPCR assay platform | Specificity 0.86 GRADE not reported 3 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-146 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled sensitivity Leave-one-out: Radovic 2018 removed | Sensitivity 1.000 GRADE not reported 2 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-146 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | HSROC area under the curve (AUC) | AUC 0.928 Low Downgraded: imprecision, inconsistency 3 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-146 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Positive predictive value (PPV) Disease prevalence 20% | PPV 0.616 [0.200, 0.200] GRADE not reported 3 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-146 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled sensitivity | Sensitivity 0.84 [0.60, 0.93] Low Downgraded: imprecision, inconsistency 3 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-146 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled sensitivity Leave-one-out: Alminana Pastor 2023 removed | Sensitivity 0.727 GRADE not reported 2 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-146 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled sensitivity qPCR assay platform | Sensitivity 0.86 GRADE not reported 3 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-146 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Negative predictive value (NPV) Disease prevalence 10% | NPV 0.983 [0.900, 0.900] GRADE not reported 3 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-146 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Sensitivity and specificity by assay platform other than qPCR Other assay platform (not qPCR) | Other assay: 0 studies; not reported No studies found 0 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-146 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Positive predictive value (PPV) Disease prevalence 5% | PPV 0.252 [0.050, 0.050] GRADE not reported 3 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-146 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled specificity Serum | Specificity 0.83 GRADE not reported 2 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-146 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled specificity Saliva | Specificity 0.87 GRADE not reported 1 study |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-146 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled sensitivity Leave-one-out: Rovas 2022 removed | Sensitivity 0.658 GRADE not reported 2 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-146 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled specificity Leave-one-out: Radovic 2018 removed | Specificity 1.000 GRADE not reported 2 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-146 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Negative predictive value (NPV) Disease prevalence 5% | NPV 0.992 [0.950, 0.950] GRADE not reported 3 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-146 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled specificity | Specificity 0.84 [0.60, 0.93] Low Downgraded: imprecision, inconsistency 3 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-146 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled specificity Leave-one-out: Rovas 2022 removed | Specificity 0.657 GRADE not reported 2 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-146 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled sensitivity Saliva | Sensitivity 0.87 GRADE not reported 1 study |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-146 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled sensitivity Serum | Sensitivity 0.85 GRADE not reported 2 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-146 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Positive predictive value (PPV) Disease prevalence 10% | PPV 0.416 [0.100, 0.100] GRADE not reported 3 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-146 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Negative predictive value (NPV) Disease prevalence 50% | NPV 0.866 [0.500, 0.500] GRADE not reported 3 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-146 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled specificity Leave-one-out: Alminana Pastor 2023 removed | Specificity 0.727 GRADE not reported 2 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-155 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled false-positive rate (labeled specificity in the source table) Leave-one-out: Dailly 2023 removed | False-positive rate 0.146 GRADE not reported 4 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-155 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Sensitivity and specificity by assay platform other than qPCR Other assay platform (not qPCR) | Other assay: 0 studies; not reported No studies found 0 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-155 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled sensitivity Plasma | Sensitivity 0.85 GRADE not reported 2 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-155 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled sensitivity Leave-one-out: Appukuttan 2025 removed | Sensitivity 0.841 GRADE not reported 4 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-155 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled sensitivity qPCR assay platform | Sensitivity 0.84 GRADE not reported 5 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-155 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled sensitivity Serum | Sensitivity 0.83 GRADE not reported 3 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-155 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled specificity qPCR assay platform | Specificity 0.88 GRADE not reported 5 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-155 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled sensitivity Leave-one-out: Baru 2025 removed | Sensitivity 0.860 GRADE not reported 4 studies |
| Adults with periodontitis (2018 AAP/EFP) and healthy controls | miR-155 expression level (index test, any specimen) | Standard clinical or radiographic periodontitis diagnosis (reference standard) | Negative predictive value Case-control comparison of periodontitis patients and healthy controls · Assumed prevalence 10% | NPV 0.980 [0.971, 0.985] GRADE not reported 5 studies |
| Adults with periodontitis (2018 AAP/EFP) and healthy controls | miR-155 expression level (index test, any specimen) | Standard clinical or radiographic periodontitis diagnosis (reference standard) | Negative predictive value Case-control comparison of periodontitis patients and healthy controls · Assumed prevalence 5% | NPV 0.990 [0.986, 0.993] GRADE not reported 5 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-155 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled false-positive rate (labeled specificity in the source table) Leave-one-out: Appukuttan 2025 removed | False-positive rate 0.094 GRADE not reported 4 studies |
| Adults with periodontitis (2018 AAP/EFP) and healthy controls | miR-155 expression level (index test, any specimen) | Standard clinical or radiographic periodontitis diagnosis (reference standard) | Negative predictive value Case-control comparison of periodontitis patients and healthy controls · Assumed prevalence 50% | NPV 0.843 [0.790, 0.879] GRADE not reported 5 studies |
| Adults with periodontitis (2018 AAP/EFP) and healthy controls | miR-155 expression level (index test, any specimen) | Standard clinical or radiographic periodontitis diagnosis (reference standard) | Positive predictive value Case-control comparison of periodontitis patients and healthy controls · Assumed prevalence 50% | PPV 0.875 [0.774, 0.935] GRADE not reported 5 studies |
| Adults with periodontitis (2018 AAP/EFP) and healthy controls | miR-155 expression level (index test, any specimen) | Standard clinical or radiographic periodontitis diagnosis (reference standard) | Negative predictive value Case-control comparison of periodontitis patients and healthy controls · Assumed prevalence 20% | NPV 0.956 [0.938, 0.967] GRADE not reported 5 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-155 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled false-positive rate (labeled specificity in the source table) Leave-one-out: Radovic 2018 removed | False-positive rate 0.146 GRADE not reported 4 studies |
| Adults with periodontitis (2018 AAP/EFP) and healthy controls | miR-155 expression level (index test, any specimen) | Standard clinical or radiographic periodontitis diagnosis (reference standard) | Positive predictive value Case-control comparison of periodontitis patients and healthy controls · Assumed prevalence 20% | PPV 0.637 [0.461, 0.782] GRADE not reported 5 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-155 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled sensitivity | Sensitivity 0.85 [0.77, 0.91] Moderate Downgraded: inconsistency 5 studies |
| Adults with periodontitis (2018 AAP/EFP) and healthy controls | miR-155 expression level (index test, any specimen) | Standard clinical or radiographic periodontitis diagnosis (reference standard) | Positive predictive value Case-control comparison of periodontitis patients and healthy controls · Assumed prevalence 5% | PPV 0.270 [0.153, 0.431] GRADE not reported 5 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-155 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled false-positive rate (labeled specificity in the source table) Leave-one-out: Baru 2025 removed | False-positive rate 0.085 GRADE not reported 4 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-155 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled sensitivity Leave-one-out: Nandipati 2022 removed | Sensitivity 0.814 GRADE not reported 4 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-155 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled sensitivity Leave-one-out: Dailly 2023 removed | Sensitivity 0.829 GRADE not reported 4 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-155 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | HSROC area under the curve (AUC) | AUC 0.903 Moderate Downgraded: inconsistency 5 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-155 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled false-positive rate (labeled specificity in the source table) Leave-one-out: Nandipati 2022 removed | False-positive rate 0.105 GRADE not reported 4 studies |
| Adults with periodontitis (2018 AAP/EFP) and healthy controls | miR-155 expression level (index test, any specimen) | Standard clinical or radiographic periodontitis diagnosis (reference standard) | Positive predictive value Case-control comparison of periodontitis patients and healthy controls · Assumed prevalence 10% | PPV 0.438 [0.276, 0.615] GRADE not reported 5 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-155 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled sensitivity Leave-one-out: Radovic 2018 removed | Sensitivity 0.850 GRADE not reported 4 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-155 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled specificity Serum | Specificity 0.88 GRADE not reported 3 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-155 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled specificity | Specificity 0.84 [0.69, 0.93] Moderate Downgraded: inconsistency 5 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-155 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled specificity Plasma | Specificity 0.87 GRADE not reported 2 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-223 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled false-positive rate (labeled specificity in the source table) Leave-one-out: Bandi 2024 removed | False-positive rate 0.236 GRADE not reported 5 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-223 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled false-positive rate (labeled specificity in the source table) Leave-one-out: AbdelKawy 2024 removed | False-positive rate 0.244 GRADE not reported 5 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-223 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled specificity qPCR assay platform | Specificity 0.77 GRADE not reported 6 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-223 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled sensitivity Leave-one-out: Bandi 2024 removed | Sensitivity 0.676 GRADE not reported 5 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-223 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled false-positive rate (labeled specificity in the source table) Leave-one-out: Bandi 2023b removed | False-positive rate 0.244 GRADE not reported 5 studies |
| Adults with periodontitis (2018 AAP/EFP) and healthy controls | miR-223 expression level (index test, any specimen) | Standard clinical or radiographic periodontitis diagnosis (reference standard) | Negative predictive value Case-control comparison of periodontitis patients and healthy controls · Assumed prevalence 20% | NPV 0.911 [0.886, 0.932] GRADE not reported 6 studies |
| Adults with periodontitis (2018 AAP/EFP) and healthy controls | miR-223 expression level (index test, any specimen) | Standard clinical or radiographic periodontitis diagnosis (reference standard) | Positive predictive value Case-control comparison of periodontitis patients and healthy controls · Assumed prevalence 50% | PPV 0.755 [0.686, 0.815] GRADE not reported 6 studies |
| Adults with periodontitis (2018 AAP/EFP) and healthy controls | miR-223 expression level (index test, any specimen) | Standard clinical or radiographic periodontitis diagnosis (reference standard) | Negative predictive value Case-control comparison of periodontitis patients and healthy controls · Assumed prevalence 50% | NPV 0.720 [0.660, 0.774] GRADE not reported 6 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-223 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled sensitivity Leave-one-out: Bandi 2023b removed | Sensitivity 0.716 GRADE not reported 5 studies |
| Adults with periodontitis (2018 AAP/EFP) and healthy controls | miR-223 expression level (index test, any specimen) | Standard clinical or radiographic periodontitis diagnosis (reference standard) | Negative predictive value Case-control comparison of periodontitis patients and healthy controls · Assumed prevalence 5% | NPV 0.980 [0.974, 0.985] GRADE not reported 6 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-223 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled sensitivity qPCR assay platform | Sensitivity 0.70 GRADE not reported 6 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-223 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Sensitivity and specificity by assay platform other than qPCR Other assay platform (not qPCR) | Other assay: 0 studies; not reported No studies found 0 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-223 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | HSROC area under the curve (AUC) | AUC 0.787 Low Downgraded: inconsistency, publication bias 6 studies |
| Adults with periodontitis (2018 AAP/EFP) and healthy controls | miR-223 expression level (index test, any specimen) | Standard clinical or radiographic periodontitis diagnosis (reference standard) | Negative predictive value Case-control comparison of periodontitis patients and healthy controls · Assumed prevalence 10% | NPV 0.959 [0.946, 0.969] GRADE not reported 6 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-223 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled sensitivity | Sensitivity 0.70 [0.55, 0.84] Low Downgraded: inconsistency, publication bias 6 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-223 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled sensitivity Saliva | Sensitivity 0.72 GRADE not reported 3 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-223 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled sensitivity Serum | Sensitivity 0.68 GRADE not reported 3 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-223 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled false-positive rate (labeled specificity in the source table) Leave-one-out: Bandi 2023a removed | False-positive rate 0.241 GRADE not reported 5 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-223 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled sensitivity Leave-one-out: Elazazy 2021 removed | Sensitivity 0.659 GRADE not reported 5 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-223 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled sensitivity Leave-one-out: Liu 2022 removed | Sensitivity 0.750 GRADE not reported 5 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-223 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled false-positive rate (labeled specificity in the source table) Leave-one-out: Liu 2022 removed | False-positive rate 0.178 GRADE not reported 5 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-223 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled sensitivity Leave-one-out: AbdelKawy 2024 removed | Sensitivity 0.698 GRADE not reported 5 studies |
| Adults with periodontitis (2018 AAP/EFP) and healthy controls | miR-223 expression level (index test, any specimen) | Standard clinical or radiographic periodontitis diagnosis (reference standard) | Positive predictive value Case-control comparison of periodontitis patients and healthy controls · Assumed prevalence 10% | PPV 0.255 [0.195, 0.329] GRADE not reported 6 studies |
| Adults with periodontitis (2018 AAP/EFP) and healthy controls | miR-223 expression level (index test, any specimen) | Standard clinical or radiographic periodontitis diagnosis (reference standard) | Positive predictive value Case-control comparison of periodontitis patients and healthy controls · Assumed prevalence 5% | PPV 0.139 [0.103, 0.189] GRADE not reported 6 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-223 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled sensitivity Leave-one-out: Bandi 2023a removed | Sensitivity 0.691 GRADE not reported 5 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-223 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled specificity | Specificity 0.77 [0.68, 0.85] Low Downgraded: inconsistency, publication bias 6 studies |
| Adults with periodontitis (2018 AAP/EFP) and healthy controls | miR-223 expression level (index test, any specimen) | Standard clinical or radiographic periodontitis diagnosis (reference standard) | Positive predictive value Case-control comparison of periodontitis patients and healthy controls · Assumed prevalence 20% | PPV 0.435 [0.353, 0.525] GRADE not reported 6 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-223 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled specificity Saliva | Specificity 0.79 GRADE not reported 3 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-223 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled false-positive rate (labeled specificity in the source table) Leave-one-out: Elazazy 2021 removed | False-positive rate 0.236 GRADE not reported 5 studies |
| Patients with periodontitis (2018 AAP/EFP classification) and periodontally healthy controls | miR-223 expression level as index test (qPCR) | Reference standard: clinical or radiographic periodontitis criteria (CAL, PPD, radiographic bone loss) | Pooled specificity Serum | Specificity 0.75 GRADE not reported 3 studies |
Included primary studies 55
Open in Studies| Study | Year | Also included by | Outcomes it informs here | Trial data |
|---|---|---|---|---|
| AbdelKawy 2024 Expression of microRNA-223 and microRNA-214 in gingival crevicular fluid of smoker and nonsmoker periodontitis patients, an observational… DOI:10.1007/s00784-024-05844-7 | 2024 | this review only | Pooled sensitivity | not extracted yet |
| AbdelKawy 2024 Expression of microRNA-223 and microRNA-214 in gingival crevicular fluid of smoker and nonsmoker periodontitis patients, an observational… PMID:39126506 | 2024 | this review only | Pooled sensitivity | not extracted yet |
| Akshaya 2025 Comparative evaluation of macrophage polarization markers- nitric oxide, sCD163 and miRNA-21 in the saliva of healthy, gingivitis and… DOI:10.1016/j.genrep.2025.102213 | 2025 | this review only | not stated | not extracted yet |
| Almiñana-Pastor 2023 MicroRNAs in Gingival Crevicular Fluid: An Observational Case-Control Study of Differential Expression in Periodontitis. PMID:37987369 | 2023 | this review only | Pooled sensitivity | not extracted yet |
| Appukuttan 2025 High Mobility Group Box-1 (HMGB1)/microRNA-155 Axis in the Pathogenesis of Type 2 Diabetes Mellitus Associated Periodontitis: A Case… DOI:10.1177/23202068241308668 | 2025 | this review only | Pooled sensitivity | not extracted yet |
| Bandi 2023a Extracellular microRNA-223-5p Levels in Plasma, Saliva, and Gingival Crevicular Fluid in Periodontal Disease as a Potential Diagnostic… DOI:10.4103/jofs.jofs_177_23 | 2023 | this review only | Pooled sensitivity | not extracted yet |
| Bandi 2023b MicroRNA-221-5p, MicroRNA-222-5p, and MicroRNA-223-5p Expression Patterns in Association with the Pathogenesis of Periodontal Disease: A… DOI:10.5005/jp-journals-10015-2310 | 2023 | this review only | Pooled sensitivity | not extracted yet |
| Bandi 2024 Expression dynamics of microRNA-223/Ras-associated binding protein 12 axis in Stage III/Grade B periodontal disease: A case-control… PMID:38988960 | 2024 | this review only | Pooled sensitivity | not extracted yet |
| Baru 2023 Identification of a miRNA Panel with a Potential Determinant Role in Patients Suffering from Periodontitis DOI:10.3390/cimb45030145 | 2023 | this review only | not stated | not extracted yet |
| Baru 2023 Identification of a miRNA Panel with a Potential Determinant Role in Patients Suffering from Periodontitis. PMID:36975515 | 2023 | this review only | not stated | not extracted yet |
| Baru 2025 The Evaluation of a 5-miRNA Panel in Patients with Periodontitis Disease DOI:10.1177/23800844241252395 | 2024 | this review only | Pooled sensitivity | not extracted yet |
| Baru 2025 matched by author and year (probable) | NR | this review only | Pooled sensitivity | not extracted yet |
| Buragaite-Staponkiene 2023 Gingival Tissue MiRNA Expression Profiling and an Analysis of Periodontitis-Specific Circulating MiRNAs DOI:10.3390/ijms241511983 | 2023 | this review only | not stated | not extracted yet |
| Buragaite-Staponkiene 2023 Gingival Tissue MiRNA Expression Profiling and an Analysis of Periodontitis-Specific Circulating MiRNAs. PMID:37569358 | 2023 | this review only | not stated | not extracted yet |
| Costantini 2023 TNFα, IL-6, miR-103a-3p, miR-423-5p, miR-23a-3p, miR-15a-5p and miR-223-3p in the crevicular fluid of periodontopathic patients correlate… DOI:10.1038/s41598-022-26421-6 | 2023 | this review only | not stated | not extracted yet |
| Costantini 2023 TNFα, IL-6, miR-103a-3p, miR-423-5p, miR-23a-3p, miR-15a-5p and miR-223-3p in the crevicular fluid of periodontopathic patients correlate… PMID:36599866 | 2023 | this review only | not stated | not extracted yet |
| Daily 2023 MicroRNA‐155 (miR-155) as an accurate biomarker of periodontal status and coronary heart disease severity: a case–control study DOI:10.1186/s12903-023-03584-w | 2023 | this review only | Pooled sensitivity | not extracted yet |
| Daily 2023 MicroRNA-155 (miR-155) as an accurate biomarker of periodontal status and coronary heart disease severity: a case-control study. PMID:37974134 | 2023 | this review only | Pooled sensitivity | not extracted yet |
| Du 2021 The expression and clinical significance of miR-1226 in patients with periodontitis DOI:10.1186/s12903-021-01855-y | 2021 | this review only | not stated | not extracted yet |
| Du 2021 The expression and clinical significance of miR-1226 in patients with periodontitis. PMID:34592963 | 2021 | this review only | not stated | not extracted yet |
| Elazazy 2021 Evaluation of serum and gingival crevicular fluid microRNA-223, microRNA-203 and microRNA-200b expression in chronic periodontitis… DOI:10.1016/j.archoralbio.2020.104949 | 2021 | this review only | Pooled sensitivity | not extracted yet |
| Elazazy 2021 Evaluation of serum and gingival crevicular fluid microRNA-223, microRNA-203 and microRNA-200b expression in chronic periodontitis… PMID:33157494 | 2021 | this review only | Pooled sensitivity | not extracted yet |
| Huang 2022 RETRACTED: MicroRNA-28-5p as a potential diagnostic biomarker for chronic periodontitis and its role in cell proliferation and… DOI:10.1016/j.jds.2022.04.031 | 2022 | this review only | not stated | not extracted yet |
| Huang 2022 MicroRNA-28-5p as a potential diagnostic biomarker for chronic periodontitis and its role in cell proliferation and inflammatory response. PMID:36299340 | 2022 | this review only | not stated | not extracted yet |
| Ibrahim 2025 matched by author and year (probable) | NR | this review only | not stated | not extracted yet |
| Jiang 2021 PMID:20512021 | NR | this review only | not stated | not extracted yet |
| Jiang 2021 miR-205 and HMGB1 expressions in chronic periodontitis patients and their associations with the inflammatory factors. PMID:34540038 | 2021 | this review only | not stated | not extracted yet |
| Li 2024 Expression Levels of miR-181 Family Members in Oral Biofluids as Biomarkers for Periodontitis Severity DOI:10.1620/tjem.2024.j058 | 2024 | this review only | not stated | not extracted yet |
| Li 2024 Expression Levels of miR-181 Family Members in Oral Biofluids as Biomarkers for Periodontitis Severity. PMID:38960640 | 2024 | this review only | not stated | not extracted yet |
| Liu 2022 Relationship between microRNA expression and inflammatory factors in patients with both type 2 diabetes mellitus and periodontal disease. PMID:36247296 | 2022 | this review only | Pooled sensitivity | not extracted yet |
| Motedayyen 2015 Evaluation of MicroRNA‐146a and Its Targets in Gingival Tissues of Patients With Chronic Periodontitis DOI:10.1902/jop.2015.150319 | 2015 | this review only | not stated | not extracted yet |
| Motedayyen 2015 Evaluation of MicroRNA-146a and Its Targets in Gingival Tissues of Patients With Chronic Periodontitis. PMID:26313020 | 2015 | this review only | not stated | not extracted yet |
| Nandipati 2022 Role of miRNA-155 in the regulation of osteoclast differentiation mediated by MITF in stage III/IV periodontitis: a case-control study DOI:10.1186/s43141-022-00441-1 | 2022 | this review only | Pooled sensitivity | not extracted yet |
| Nandipati 2022 Role of miRNA-155 in the regulation of osteoclast differentiation mediated by MITF in stage III/IV periodontitis: a case-control study. PMID:36459254 | 2022 | this review only | Pooled sensitivity | not extracted yet |
| Ni 2023 Non-invasive diagnostic potential of salivary miR-25-3p for periodontal disease and osteoporosis among a cohort of elderly patients with… DOI:10.1186/s12903-023-02992-2 | 2023 | this review only | not stated | not extracted yet |
| Ni 2023 Non-invasive diagnostic potential of salivary miR-25-3p for periodontal disease and osteoporosis among a cohort of elderly patients with… PMID:37221590 | 2023 | this review only | not stated | not extracted yet |
| Radovic 2018 MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in non-diabetic and type 2 diabetic patients. PMID:29517812 | 2018 | this review only | Pooled sensitivity | not extracted yet |
| Radović 2018 Micro<scp>RNA</scp>‐146a and micro<scp>RNA</scp>‐155 as novel crevicular fluid biomarkers for periodontitis in non‐diabetic and type 2… DOI:10.1111/jcpe.12888 | 2018 | this review only | Pooled sensitivity | not extracted yet |
| Rovas 2021 Analysis of periodontitis-associated miRNAs in gingival tissue, gingival crevicular fluid, saliva and blood plasma DOI:10.1016/j.archoralbio.2021.105125 | 2021 | this review only | not stated | not extracted yet |
| Rovas 2021 Analysis of periodontitis-associated miRNAs in gingival tissue, gingival crevicular fluid, saliva and blood plasma. PMID:33862403 | 2021 | this review only | not stated | not extracted yet |
| Rovas 2022 Gingival crevicular fluid microRNA associations with periodontitis DOI:10.2334/josnusd.21-0282 | 2022 | this review only | Pooled sensitivity | not extracted yet |
| Rovas 2022 Gingival crevicular fluid microRNA associations with periodontitis. PMID:34690249 | 2022 | this review only | Pooled sensitivity | not extracted yet |
| Wu 2021 Expression of miR-155 and miR-146a in the saliva of patients with periodontitis and its clinical value. PMID:34306411 | 2021 | this review only | not stated | not extracted yet |
| Wu 2022 Correlation Analysis of miR-1246 Expression in Saliva of Patients with Chronic Periodontitis and Periodontal Indexes, Inflammatory… DOI:10.1155/2022/1949159 | 2022 | this review only | not stated | not extracted yet |
| Wu 2022 Correlation Analysis of miR-1246 Expression in Saliva of Patients with Chronic Periodontitis and Periodontal Indexes, Inflammatory… PMID:35942384 | 2022 | this review only | not stated | not extracted yet |
| Yagnik 2019 The Periodontal‐Cardiovascular alliance: Evaluation of miRNA‐146a in subgingival plaque samples of chronic periodontitis patients with and… DOI:10.1111/jicd.12442 | 2019 | this review only | not stated | not extracted yet |
| Yagnik 2019 The Periodontal-Cardiovascular alliance: Evaluation of miRNA-146a in subgingival plaque samples of chronic periodontitis patients with and… PMID:31338994 | 2019 | this review only | not stated | not extracted yet |
| Yan 2025 Clinical significance analysis of microRNA-199a-3p in gingival crevicular fluid for patients with chronic periodontitis DOI:10.1016/j.mcp.2025.102015 | 2025 | this review only | not stated | not extracted yet |
| Yan 2025 Clinical significance analysis of microRNA-199a-3p in gingival crevicular fluid for patients with chronic periodontitis. PMID:39870183 | 2025 | this review only | not stated | not extracted yet |
| Yoneda 2019 Serum microRNAs and chronic periodontitis: A case-control study DOI:10.1016/j.archoralbio.2019.03.009 | 2019 | this review only | not stated | not extracted yet |
| Yoneda 2019 Serum microRNAs and chronic periodontitis: A case-control study. PMID:30889506 | 2019 | this review only | not stated | not extracted yet |
| Yu 2023 Diagnostic potential of miR-200 family members in gingival crevicular fluid for chronic periodontitis: correlation with clinical… DOI:10.1186/s12903-023-03174-w | 2023 | this review only | not stated | not extracted yet |
| Yu 2023 Diagnostic potential of miR-200 family members in gingival crevicular fluid for chronic periodontitis: correlation with clinical… PMID:37525201 | 2023 | this review only | not stated | not extracted yet |
| Zhu 2022 The expression and clinical significance of miR-30b-3p and miR-125b-1-3p in patients with periodontitis DOI:10.1186/s12903-022-02360-6 | 2022 | this review only | not stated | not extracted yet |
| Zhu 2022 The expression and clinical significance of miR-30b-3p and miR-125b-1-3p in patients with periodontitis. PMID:35931990 | 2022 | this review only | not stated | not extracted yet |
Review question
| Element | Planned (methods) | Found (results) |
|---|---|---|
| Population | Patients diagnosed with periodontitis (2018 AAP/EFP classification) and healthy controls | Periodontitis patients and healthy controls (n=1,177 across 14 studies in the meta-analysis); 6 studies with diabetes, 2 with coronary heart disease |
| Intervention | Analysis of miRNA expression associated with periodontitis (index test) | miR-146 (3 studies), miR-155 (5 studies), miR-223 (6 studies) expression in gingival crevicular fluid, saliva, serum, plasma, gingival tissue (all qPCR) |
| Comparison | Standard clinical or radiographic diagnostic criteria for periodontitis (CAL, PPD, radiographic bone loss) | Clinical or radiographic periodontitis diagnosis versus healthy controls |
| Outcomes | Diagnostic accuracy of miRNAs: sensitivity, specificity and area under the curve (AUC) | Pooled sensitivity, specificity, HSROC AUC, PPV and NPV |
| Study designs | Observational human studies (cross-sectional, case-control, cohort) with >=10 participants per group | 32 studies in the review (21 case-control, 1 prospective cohort, 1 retrospective case-control, 9 cross-sectional); 14 in meta-analysis |
| Timing | Time of miRNA sample collection relative to disease status or clinical diagnosis (if reported) | Not specified |