Non-Cochrane · prognostic · PM38233799
Gene sequencing applications to combat oral-cavity related disorders: a systematic review with meta-analysis.
Abdul Nishath Sayed, Shenoy Mahesh, Reddy Naveen Rami, et al. · BMC oral health · 2024 · PubMed · doi:10.1186/s12903-023-03541-7
Full textEvery question was extracted from the full text, figures and supplements by two independent LLM extractors, disagreements adjudicated. Rows cite where in the review the value comes from.
Questions
3
current version
GRADE rated
0
No studies
0
Included studies
8
linked below
No studies 0 · 0%Ungraded 3 · 100%Very low 0 · 0%Low 0 · 0%Moderate 0 · 0%High 0 · 0%
Ungraded means an effect was reported without a GRADE rating; it is not a gap. No studies means the review found nothing to answer the question. The six categories are a display order, not a score.
Review question
| Element | Planned (methods) | Found (results) |
|---|---|---|
| Population | Human subjects of any age, gender, ethnicity, and geographical location with oral-cavity related disorders (dental caries, periodontitis, gingivitis, oral cancer, among others) | Patients with various oral-cavity disorders (dental caries, periodontitis, gingivitis, oral cancer, cleft lip, peri-implant mucositis) across 9 included studies (n=9 studies; 8 clinical trials meta-analyzed, 2211 participants) |
| Intervention | Gene sequencing techniques: next-generation sequencing (NGS), single-molecule sequencing, whole-exome and whole-genome sequencing; RNA-seq (mRNA, small RNA, non-coding RNA) | Gene sequencing, predominantly 16S rRNA next-generation sequencing of the oral microbiome; one genome-wide association study (cleft lip) (n=9 studies) |
| Comparison | Not specified (no comparator defined in the eligibility criteria) | No distinct comparator arm; forest plots contrast 'noticeable impact' vs 'abysmal effect' events within the same samples |
| Outcomes | Impact of gene sequencing on diagnosing and/or treating oral-cavity disorders; gene-expression profiles related to oral diseases | Noticeable impact of gene sequencing on diagnosing/managing oral conditions, pooled as OR, RR and RD (n=8 studies meta-analyzed) |
| Study designs | Not specified (peer-reviewed studies; synthesis later restricted to clinical trials) | 9 studies: 6 randomized controlled trials, 2 observational trials, and 1 literature review (8 clinical trials entered the meta-analysis) |
| Timing | Not specified | Varies by study (e.g. baseline to 1-3 months in microbiome studies); not uniformly reported |
Search date: Not reported (search restricted to studies published 2015 to 2022; exact search-run date not given). Databases: PubMed; Scopus; Embase; Web of Science; Google Scholar
Questions and results
Gene sequencing (GS) applied to diagnose/manage oral conditions (predominantly 16S rRNA next-generation sequencing of the oral microbiome; also genome-wide/whole-genome sequencing) vs Negligible ('abysmal') effect of gene sequencing; no separate comparator arm (within-sample event contrast in the forest plots)
Population: Human patients (any age) with oral-cavity related disorders (e.g. dental caries, periodontitis, gingivitis, peri-implant mucositis, oral/head-and-neck cancer, cleft lip)
| Outcome | Effect | Evidence | Status / GRADE |
|---|---|---|---|
Noticeable impact of gene sequencing on diagnosing/managing oral conditions Overall Pooled across 8 clinical trials, gene sequencing had a statistically significant impact on diagnosing/managing oral-cavity disorders (RR 0.50, 95% CI 0.46-0.54); certainty not GRADE-rated. Source: Fig. 4 (risk-ratio forest plot); Results (forest-plot description) · same question in other reviews | RR 0.50 [0.46, 0.54] | 8 studies · 2211 participants | Ungraded |
Noticeable impact of gene sequencing on diagnosing/managing oral conditions Overall Pooled across 8 clinical trials, gene sequencing had a statistically significant impact on diagnosing/managing oral-cavity disorders (OR 0.30, 95% CI 0.26-0.33); certainty not GRADE-rated. Source: Fig. 3 (odds-ratio forest plot); Results (Statistical analysis; forest-plot description) · same question in other reviews | OR 0.30 [0.26, 0.33] | 8 studies · 2211 participants | Ungraded |
Reduction in occurrence of oral conditions Overall Pooled across 8 clinical trials, gene sequencing was associated with a statistically significant risk difference for impact on oral-cavity disorders (RD -0.29, 95% CI -0.32 to -0.26); certainty not GRADE-rated. Source: Results (text description of Fig. 5, risk-difference forest plot) · same question in other reviews | RD -0.29 [-0.32, -0.26] | 8 studies · 2211 participants | Ungraded |
Included primary studies 8
Open as a trial list| Study | Also included by | Outcomes it informs here | Trial PICO | Results |
|---|---|---|---|---|
| Benic et al. 2019 Oral probiotics reduce halitosis in patients wearing orthodontic braces: a randomized, triple-blind, placebo-controlled trial. PMID:31022704 | this review only | Reduction in occurrence of oral conditions | not extracted yet | not extracted yet |
| De Oliveira et al. 2021 Impact of systemic probiotics as adjuncts to subgingival instrumentation on the oral-gut microbiota associated with periodontitis: A… PMID:34028826 | this review only | Reduction in occurrence of oral conditions | not extracted yet | not extracted yet |
| Divaris et al. 2019 The Era of the Genome and Dental Medicine. PMID:31329043 | this review only | not stated | not extracted yet | not extracted yet |
| Ihara et al. 2019 Identification of Initial Colonizing Bacteria in Dental Plaques from Young Adults Using Full-Length 16S rRNA Gene Sequencing. PMID:31481603 | this review only | Reduction in occurrence of oral conditions | not extracted yet | not extracted yet |
| Kao et al. 2022 Afatinib and Pembrolizumab for Recurrent or Metastatic Head and Neck Squamous Cell Carcinoma (ALPHA Study): A Phase II Study with… PMID:35046059 | this review only | Reduction in occurrence of oral conditions | not extracted yet | not extracted yet |
| Philip et al. 2022 The microbiome of dental and peri-implant subgingival plaque during peri-implant mucositis therapy: A randomized clinical trial. PMID:34664294 | this review only | Reduction in occurrence of oral conditions | not extracted yet | not extracted yet |
| Van et al. 2019 Non-Syndromic Cleft Lip with or without Cleft Palate: Genome-Wide Association Study in Europeans Identifies a Suggestive Risk Locus at… PMID:31817908 | this review only | Reduction in occurrence of oral conditions | not extracted yet | not extracted yet |
| Zaura et al. 2015 Same Exposure but Two Radically Different Responses to Antibiotics: Resilience of the Salivary Microbiome versus Long-Term Microbial… PMID:26556275 | this review only | Reduction in occurrence of oral conditions | not extracted yet | not extracted yet |