Non-Cochrane · other · PM41624011
Specific gene expression patterns associated as reliable biomarkers for predicting dental implant successful osseointegration: a literature review and focused meta-analysis.
Serrato-Pedrosa Jesus Alejandro, Bocanegra-García Virgilio, Villanueva-Fierro Ignacio, et al. · Frontiers in physiology · 2025 · PubMed · doi:10.3389/fphys.2025.1682440
Findings
6 findings
| Population | ||||
|---|---|---|---|---|
| In vitro osteogenic cell models of dental implant osseointegration | Modified titanium implant surface | Control (unmodified) titanium surface | RUNX2 gene expression (relative expression) In vitro laboratory Unresolved: the two extractors disagree on this row. | SMD 5.38 [0.66, 10.1] Low Downgraded: risk of bias, inconsistency, indirectness 4 studies · 32 participants |
| In vitro osteogenic cell models of dental implant osseointegration | Modified titanium implant surface | Control (unmodified) titanium surface | RUNX2 gene expression (relative expression) In vitro laboratory · Estimate as reported in the abstract (differs from Figure 2) Unresolved: the two extractors disagree on this row. | SMD 2.58 [1.21, 3.95] GRADE not reported Studies not reported |
| In vitro, animal and human studies of pro-osteogenic markers on implant surfaces | Modified implant surface | Control surface | Upregulation of pro-osteogenic markers (RUNX2) GRADE summary, all 13 primary studies Unresolved: the two extractors disagree on this row. | Not applicable (13 primary studies, including 6 in vitro studies in meta-analysis); LOW Low Downgraded: risk of bias, imprecision, inconsistency, indirectness 13 studies |
| Dental implant failure and peri-implantitis (clinical, animal and in vitro studies) | Increased RANKL/OPG ratio | Successful osseointegration / normal ratio | Implant failure (association with RANKL/OPG ratio) | Table 3: 3 primary studies (clinical, animal, in vitro); Low certainty; upgraded one level for large effect (20-fold decrease in OPG) Low Downgraded: inconsistency, indirectness 3 studies |
| Dental implant failure and peri-implantitis (clinical, animal and in vitro studies) | Elevated pro-inflammatory cytokines (IL-1B, IL-6, TNF-alpha) | Successful osseointegration / normal expression | Implant failure (association with cytokine expression) | Table 3: 11 primary studies (clinical, animal, in vitro); Low certainty; upgraded one level for large effect (20-fold increase in CCL5) Low Downgraded: risk of bias, inconsistency, indirectness 11 studies |
| In vitro and animal models of dental implant osseointegration | Implant surface modifications | Control or standard surfaces | Modulation of key signaling pathways (NF-kB, Wnt) | Table 3: 7 primary studies (in vitro, animal); Very Low certainty Very low Downgraded: risk of bias, inconsistency, indirectness 7 studies |
Included primary studies 60
Open in Studies| Study | Year | Also included by | Outcomes it informs here | Trial data |
|---|---|---|---|---|
| Manor 2021 A retrospective analysis of dental implantation under anticoagulant treatment. PMID:32506326 | 2021 | 1 other review | not stated | not extracted yet |
| Manor et al. (2021) A retrospective analysis of dental implantation under anticoagulant treatment DOI:10.1007/s00784-020-03389-z | 2020 | 1 other review | not stated | not extracted yet |
| Alkhoury 2020 Studies on the Mechanisms of Anti-Inflammatory Activity of Heparin- and Hyaluronan-Containing Multilayer Coatings-Targeting NF-κB… PMID:32466274 | 2020 | this review only | not stated | not extracted yet |
| Bayandurov 2024 Selected osteointegration markers in different timeframes after dental implantation: findings and prognostic value. PMID:38721304 | 2024 | this review only | not stated | not extracted yet |
| Bayandurov et al. (2024) Selected osteointegration markers in different timeframes after dental implantation: findings and prognostic value DOI:10.3389/abp.2024.12433 | 2024 | this review only | not stated | not extracted yet |
| Benalcazar-Jalkh 2023 Impact of implant thread design on insertion torque and osseointegration: a preclinical model. PMID:36173722 | 2023 | this review only | not stated | not extracted yet |
| Cirera 2020 Osseointegration around dental implants biofunctionalized with TGFβ-1 inhibitor peptides: an in vivo study in beagle dogs. PMID:32696084 | 2020 | this review only | not stated | not extracted yet |
| Cirera et al. (2020) Osseointegration around dental implants biofunctionalized with TGFβ-1 inhibitor peptides: an in vivo study in beagle dogs DOI:10.1007/s10856-020-06397-3 | 2020 | this review only | not stated | not extracted yet |
| da Cruz 2022 Laser surface treatment on Yttria-stabilized zirconia dental implants: Influence on cell behavior. PMID:34278714 | 2022 | this review only | not stated | not extracted yet |
| da Cruz et al. (2022) Laser surface treatment on Yttria‐stabilized zirconia dental implants: Influence on cell behavior DOI:10.1002/jbm.b.34909 | 2021 | this review only | not stated | not extracted yet |
| dos Santos Trento 2020 Gene expression, immunohistochemical and microarchitectural evaluation of bone formation around two implant surfaces placed in bone… PMID:33258065 | 2020 | this review only | not stated | not extracted yet |
| dos Santos Trento et al. (2020) Gene expression, immunohistochemical and microarchitectural evaluation of bone formation around two implant surfaces placed in bone… DOI:10.1186/s40729-020-00279-7 | 2020 | this review only | not stated | not extracted yet |
| Gil 2022 The effect of using Al2O3 and TiO2 in sandblasting of titanium dental implants DOI:10.1557/s43578-022-00534-0 | 2022 | this review only | not stated | not extracted yet |
| Gnanajothi 2024 Inflammatory Status of Patients with Dental Implants of Different Microgeometry using ELISA: A Prospective Clinical Study DOI:10.4103/jioh.jioh_47_24 | 2024 | this review only | not stated | not extracted yet |
| Han 2025a Development of Zinc-Containing Chitosan/Gelatin Coatings with Immunomodulatory Effect for Soft Tissue Sealing around Dental Implants. PMID:39579168 | 2025 | this review only | not stated | not extracted yet |
| Han et al. (2025a) matched by author and year (probable) | NR | this review only | not stated | not extracted yet |
| Hergem et al. (2022) Osteocalcin and Runx2 Expression in Anterior Maxillary Reconstructions Using Bone Xenografts Associated to Bone Marrow Aspirate Concentrate DOI:10.4103/ccd.ccd_723_20 | 2022 | this review only | not stated | not extracted yet |
| Hergemoller 2022 Osteocalcin and Runx2 Expression in Anterior Maxillary Reconstructions Using Bone Xenografts Associated to Bone Marrow Aspirate Concentrate. PMID:36213846 | 2022 | this review only | not stated | not extracted yet |
| Irandoust 2020 The interplay between bone healing and remodeling around dental implants. PMID:32152332 | 2020 | this review only | not stated | not extracted yet |
| Irandoust and Müftü (2020) The interplay between bone healing and remodeling around dental implants DOI:10.1038/s41598-020-60735-7 | 2020 | this review only | not stated | not extracted yet |
| Jung 2021 The Effects of NF-kB Inhibition with p65-TMD-Linked PTD on Inflammatory Responses at Peri-implantitis Sites. PMID:34169410 | 2021 | this review only | not stated | not extracted yet |
| Jung et al. (2021) The Effects of NF-kB Inhibition with p65-TMD-Linked PTD on Inflammatory Responses at Peri-implantitis Sites DOI:10.1007/s10753-021-01500-4 | 2021 | this review only | not stated | not extracted yet |
| Komatsu 2025 Advancing osseointegration research: A dynamic three-dimensional (3D) in vitro culture model for dental implants. PMID:39873044 | 2025 | this review only | not stated | not extracted yet |
| Komatsu et al. (2025) Advancing osseointegration research: A dynamic three-dimensional (3D) in vitro culture model for dental implants DOI:10.1016/j.jds.2024.06.018 | 2025 | this review only | not stated | not extracted yet |
| Le 2021 Bioactivation Treatment with Mixed Acid and Heat on Titanium Implants Fabricated by Selective Laser Melting Enhances Preosteoblast Cell… PMID:33921268 | 2021 | this review only | Upregulation of pro-osteogenic markers (RUNX2) | not extracted yet |
| Le et al. (2021) Bioactivation Treatment with Mixed Acid and Heat on Titanium Implants Fabricated by Selective Laser Melting Enhances Preosteoblast Cell… DOI:10.3390/nano11040987 | 2021 | this review only | Upregulation of pro-osteogenic markers (RUNX2) | not extracted yet |
| Lechner 2024 Osseointegration and osteoimmunology in implantology: assessment of the immune sustainability of dental implants using advanced… PMID:38259068 | 2024 | this review only | not stated | not extracted yet |
| Lechner et al. (2024) Osseointegration and osteoimmunology in implantology: assessment of the immune sustainability of dental implants using advanced… DOI:10.1177/03000605231224161 | 2024 | this review only | not stated | not extracted yet |
| Maghsoudi 2022 Bone remodeling around dental implants after 1-1.5 years of functional loading: A retrospective analysis of two-stage implants. PMID:35427440 | 2022 | this review only | not stated | not extracted yet |
| Maghsoudi et al. (2022) Bone remodeling around dental implants after 1–1.5 years of functional loading: A retrospective analysis of two‐stage implants DOI:10.1002/cre2.574 | 2022 | this review only | not stated | not extracted yet |
| Mukaddam 2025 TNF-α Activates NF-κB Signalling Pathway in MG-63 Cells on Titanium and Zirconia Implant Surfaces. PMID:40004407 | 2025 | this review only | not stated | not extracted yet |
| Mukaddam et al. (2025) TNF-α Activates NF-κB Signalling Pathway in MG-63 Cells on Titanium and Zirconia Implant Surfaces DOI:10.3390/ma18040884 | 2025 | this review only | not stated | not extracted yet |
| Palermo 2022 Use of CGF in Oral and Implant Surgery: From Laboratory Evidence to Clinical Evaluation. PMID:36499489 | 2022 | this review only | Upregulation of pro-osteogenic markers (RUNX2) | not extracted yet |
| Palermo et al. (2022) Use of CGF in Oral and Implant Surgery: From Laboratory Evidence to Clinical Evaluation DOI:10.3390/ijms232315164 | 2022 | this review only | Upregulation of pro-osteogenic markers (RUNX2) | not extracted yet |
| Romero-Gavilan 2023 Proteomic evaluation of human osteoblast responses to titanium implants over time. PMID:36054528 | 2023 | this review only | not stated | not extracted yet |
| Romero-Gavilán et al. (2023) Proteomic evaluation of human osteoblast responses to titanium implants over time DOI:10.1002/jbm.a.37444 | 2022 | this review only | not stated | not extracted yet |
| Roy 2024 Biocompatibility of Subperiosteal Dental Implants: Changes in the Expression of Osteogenesis-Related Genes in Osteoblasts Exposed to… PMID:38921520 | 2024 | this review only | not stated | not extracted yet |
| Roy et al. (2024) Biocompatibility of Subperiosteal Dental Implants: Changes in the Expression of Osteogenesis-Related Genes in Osteoblasts Exposed to… DOI:10.3390/jfb15060146 | 2024 | this review only | not stated | not extracted yet |
| Sadrkhah 2023 An In-Vivo Study on Nanostructured Ti Dental Implant Produced by Caliber Rolling and Surface Modification by SLActive DOI:10.1007/s11837-023-06050-8 | 2023 | this review only | not stated | not extracted yet |
| Schluessel 2022 Dental and Orthopaedic Implant Loosening: Overlap in Gene Expression Regulation. PMID:35222398 | 2022 | this review only | not stated | not extracted yet |
| Schluessel et al. (2022) Dental and Orthopaedic Implant Loosening: Overlap in Gene Expression Regulation DOI:10.3389/fimmu.2022.820843 | 2022 | this review only | not stated | not extracted yet |
| Shin 2022 Enhanced osseointegration of dental implants with reduced graphene oxide coating. PMID:35313996 | 2022 | this review only | Upregulation of pro-osteogenic markers (RUNX2) | not extracted yet |
| Shin et al. (2022) Enhanced osseointegration of dental implants with reduced graphene oxide coating DOI:10.1186/s40824-022-00257-7 | 2022 | this review only | Upregulation of pro-osteogenic markers (RUNX2) | not extracted yet |
| Tang 2021 Role of the Hippo-YAP/NF-κB signaling pathway crosstalk in regulating biological behaviors of macrophages under titanium ion exposure. PMID:33058278 | 2021 | this review only | not stated | not extracted yet |
| Tavelli 2024 Characterization of oral biomarkers during early healing at augmented dental implant sites. PMID:39090529 | 2025 | this review only | not stated | not extracted yet |
| Tavelli et al. (2023) Wound healing dynamics, morbidity, and complications of palatal soft‐tissue harvesting DOI:10.1111/prd.12466 | 2022 | this review only | not stated | not extracted yet |
| Tavelli et al. (2024) Characterization of oral biomarkers during early healing at augmented dental implant sites DOI:10.1111/jre.13328 | 2024 | this review only | not stated | not extracted yet |
| Valente 2021 Collagen Fibres Orientation in the Bone Matrix around Dental Implants: Does the Implant's Thread Design Play a Role? PMID:34360630 | 2021 | this review only | not stated | not extracted yet |
| Valente et al. (2021) Collagen Fibres Orientation in the Bone Matrix around Dental Implants: Does the Implant’s Thread Design Play a Role? DOI:10.3390/ijms22157860 | 2021 | this review only | not stated | not extracted yet |
| Wang 2020 Flame-sprayed strontium- and magnesium-doped hydroxyapatite on titanium implants for osseointegration enhancement DOI:10.1016/j.surfcoat.2020.125452 | 2020 | this review only | Upregulation of pro-osteogenic markers (RUNX2) | not extracted yet |
| Wang 2021a Potential biomarkers of abnormal osseointegration of implants in type II diabetes mellitus. PMID:34794414 | 2021 | this review only | not stated | not extracted yet |
| Wang 2021b Expression Level and Clinical Significance of Inflammatory Cytokines and Biochemical Markers in Gingival Crevicular Fluid During Different… PMID:34397028 | 2021 | this review only | not stated | not extracted yet |
| Wang et al. (2021a) matched by author and year (probable) | NR | this review only | not stated | not extracted yet |
| Wang et al. (2021b) matched by author and year (probable) | NR | this review only | not stated | not extracted yet |
| Wu 2021 Melatonin prevents peri‑implantitis via suppression of TLR4/NF-κB. PMID:34271168 | 2021 | this review only | not stated | not extracted yet |
| Wu et al. (2021) Melatonin prevents peri‑implantitis via suppression of TLR4/NF-κB DOI:10.1016/j.actbio.2021.07.017 | 2021 | this review only | not stated | not extracted yet |
| Zhang 2021a Mechanical properties and osteoblast proliferation of complex porous dental implants filled with magnesium alloy based on 3D printing. PMID:32915665 | 2021 | this review only | not stated | not extracted yet |
| Zhang 2023a Improving the osseointegration and soft tissue sealing of zirconia ceramics by the incorporation of akermanite via sol infiltration for… PMID:37115523 | 2023 | this review only | not stated | not extracted yet |
| Zhang et al. (2021a) matched by author and year (probable) | NR | this review only | not stated | not extracted yet |
| Zhang et al. (2023a) matched by author and year (probable) | NR | this review only | not stated | not extracted yet |
Overlap with other reviews
Reviews that include the same primary studies are not independent evidence. Shared counts match studies by PMID, DOI or NCT, and otherwise by author and year (a probable match). Only the non-Cochrane dental reviews have study lists, so overlap with Cochrane reviews is not counted yet.
| Review | Shared studies | Jaccard |
|---|---|---|
| Postoperative Bleeding Complications Associated With Dental Implant Placement in Patients Receiving Antithrombotic Therapy: A Systematic… (2025) | 2 | 0.02 |
Review question
| Element | Planned (methods) | Found (results) |
|---|---|---|
| Population | In vitro (cell lines, organoids), in vivo (animal models) and human studies related to dental implant osseointegration | In vitro, animal and human studies (n=32 studies) |
| Intervention | Assessment of gene expression patterns, biomarkers or molecular signaling pathways towards osseointegration | Gene expression biomarkers and signaling pathways; implant surface modifications |
| Comparison | Baseline or traditional understanding of osseointegration vs new technologies such as surface modifications | Control or traditional implant surfaces |
| Outcomes | Markers of successful osseointegration (e.g. RUNX2 upregulation) or implant failure (inflammatory cytokines, RANKL/OPG imbalance) | RUNX2 and other pro-osteogenic markers; pro-inflammatory cytokines (IL-1b, IL-6, TNF-a); RANKL/OPG ratio; signaling pathways (NF-kB, Wnt) |
| Study designs | Primary research: in vitro, in vivo animal, prospective/retrospective clinical studies, January 2020 to May 2025, English | In vitro, animal and clinical studies (n=32); meta-analysis of 4 in vitro studies |
| Timing | Not specified | Not specified |