Non-Cochrane · intervention · PM41487705
Systematic review and meta-analysis on the effect of olive oil in the treatment of periodontal diseases.
López-Valverde Nansi, López-Valverde Antonio, Blanco Rueda José A · Frontiers in oral health · 2025 · PubMed · doi:10.3389/froh.2025.1735845
Findings
12 findings
| Population | ||||
|---|---|---|---|---|
| Adults with periodontitis receiving non-surgical periodontal therapy | Ozonated olive oil (OzOLO) as single or adjunctive treatment | Conventional treatment, placebo or no treatment | CAL gain 8-12 weeks | SMD -0.26 [-1.08, 0.56] GRADE not reported 3 studies · 120 participants |
| Adults with periodontitis receiving non-surgical periodontal therapy | Ozonated olive oil (OzOLO) as single or adjunctive treatment | Conventional treatment, placebo or no treatment | Bleeding on probing (BoP) reduction 3-4 weeks | SMD -0.32 [-0.77, 0.13] GRADE not reported 3 studies · 196 participants |
| Adults with periodontitis receiving non-surgical periodontal therapy | Ozonated olive oil (OzOLO) as single or adjunctive treatment | Conventional treatment, placebo or no treatment | PPD reduction 8-12 weeks | SMD -0.95 [-5.30, 3.40] GRADE not reported 3 studies · 140 participants |
| Adults with periodontitis receiving non-surgical periodontal therapy | Ozonated olive oil (OzOLO) as single or adjunctive treatment | Conventional treatment, placebo or no treatment | Bleeding on probing (BoP) reduction 8-12 weeks | SMD -0.66 [-1.07, -0.26] GRADE not reported 2 studies · 100 participants |
| Adults with periodontitis receiving non-surgical periodontal therapy | Ozonated olive oil (OzOLO) as single or adjunctive treatment | Conventional treatment, placebo or no treatment | CAL gain 12-24 weeks | SMD -0.87 [-3.00, 1.26] GRADE not reported 2 studies · 40 participants |
| Adults with periodontitis receiving non-surgical periodontal therapy | Ozonated olive oil (OzOLO) as single or adjunctive treatment | Conventional treatment, placebo or no treatment | CAL gain 3-4 weeks | SMD 0.13 [-0.72, 0.98] GRADE not reported 3 studies · 120 participants |
| Adults with periodontitis receiving non-surgical periodontal therapy | Ozonated olive oil (OzOLO) as single or adjunctive treatment | Conventional treatment, placebo or no treatment | Bleeding on probing (BoP) reduction 12-24 weeks | SMD -0.22 [-0.63, 0.19] GRADE not reported 2 studies · 116 participants |
| Adults with periodontitis receiving non-surgical periodontal therapy | Ozonated olive oil (OzOLO) as single or adjunctive treatment | Conventional treatment, placebo or no treatment | PPD reduction 12-24 weeks | SMD -1.12 [-3.02, 0.78] GRADE not reported 2 studies · 116 participants |
| Adults with periodontitis receiving non-surgical periodontal therapy | Ozonated olive oil (OzOLO) as single or adjunctive treatment | Conventional treatment, placebo or no treatment | PPD reduction 3-4 weeks | SMD 0.03 [-0.80, 0.86] GRADE not reported 5 studies · 256 participants |
| Adults with gingivitis | Olive oil (OLO) or ozonated olive oil (OzOLO) | Conventional treatment, placebo or no treatment | Combined gingival indices (PI, BI, GI) reduction 2-8 weeks | SMD -1.52 [-2.60, -0.44] GRADE not reported 3 studies · 230 participants |
| Adults with gingivitis | Olive oil (OLO) or ozonated olive oil (OzOLO) | Conventional treatment, placebo or no treatment | Bleeding index (BI) reduction 2-8 weeks | SMD -0.35 [-1.42, 0.71] GRADE not reported 2 studies · 91 participants |
| Adults with gingivitis | Olive oil (OLO) or ozonated olive oil (OzOLO) | Conventional treatment, placebo or no treatment | Plaque index (PI) reduction 2-8 weeks | SMD -1.77 [-3.66, 0.12] GRADE not reported 3 studies · 115 participants |
Included primary studies 12
Open in Studies| Study | Year | Also included by | Outcomes it informs here | Trial data |
|---|---|---|---|---|
| Patel 2012 Effect of subgingival application of topical ozonated olive oil in the treatment of chronic periodontitis: a randomized, controlled,… PMID:22976566 | 2012 | 3 other reviews | not stated | not extracted yet |
| Nambiar 2022 Comparison of Ozonated Olive Oil and Chlorhexidine Gel as an Adjunct to Nonsurgical Periodontal Therapy for the Treatment of Chronic… PMID:36110593 | 2022 | 2 other reviews | not stated | not extracted yet |
| Nardi 2020 Evaluation of Salivary Matrix Metalloproteinase (MMP-8) in Periodontal Patients Undergoing Non-Surgical Periodontal Therapy and Mouthwash… PMID:32932898 | 2020 | 2 other reviews | not stated | not extracted yet |
| Mukherjee 2024 Efficacy of Olive Oil, Ozonated Olive Oil, and Chlorhexidine Gel as an Adjunct to Scaling and Root Planing in Chronic Periodontitis: A… PMID:39926739 | 2024 | 1 other review | not stated | not extracted yet |
| Patel 2025 Evaluating Ozonated Olive Oil and Chlorhexidine Gel as a Therapeutic Intervention for Periodontitis Stage II Grade A. PMID:40585734 | 2025 | 1 other review | not stated | not extracted yet |
| Indurkar 2016 Effect of ozonated oil and chlorhexidine gel on plaque induced gingivitis: A randomized control clinical trial. PMID:27041835 | 2016 | this review only | not stated | not extracted yet |
| Khare 2019 Comparative evaluation of effects of subgingival application of ozonated olive oil versus chlorhexidine in patients with chronic… DOI:10.18231/j.jds.2019.023 | 2020 | this review only | not stated | not extracted yet |
| Meenakshi 2024 Effectiveness of Microneedling Technique Using Olive Oil on the Severity of Gingival Inflammation and Plaque Accumulation: A Randomised… PMID:38826610 | 2024 | this review only | not stated | not extracted yet |
| Rodríguez-Agurto 2023 Randomized clinical trial on the clinical effects of a toothpaste containing extra virgin olive oil, xylitol, and betaine in gingivitis. PMID:37072503 | 2023 | this review only | not stated | not extracted yet |
| Vijeyakumar 2022 The Effects of Sub Gingival Application of Ozonated Olive Oil Gelatin Film in Patients with Chronic Periodontitis DOI:10.52403/ijrr.20220503 | 2022 | this review only | not stated | not extracted yet |
| Yadav 2025 DOI:10.69605/ijlbpr_14.8.2025.65 | NR | this review only | not stated | not extracted yet |
| Zumbo 2023 Oil Pulling and Polyphenols: Treatment of Gingivitis Patients with 'Itri Extra-Virgin Olive Oil'. PMID:37629298 | 2023 | this review only | not stated | not extracted yet |
Overlap with other reviews
Reviews that include the same primary studies are not independent evidence. Shared counts match studies by PMID, DOI or NCT, and otherwise by author and year (a probable match). Only the non-Cochrane dental reviews have study lists, so overlap with Cochrane reviews is not counted yet.
Review question
| Element | Planned (methods) | Found (results) |
|---|---|---|
| Population | Adults (18 years or older) with periodontal disease (gingivitis or periodontitis) | Periodontitis (7 trials, 236 subjects) and gingivitis (5 trials, 220 subjects); 456 subjects in total |
| Intervention | Olive oil (OLO) or ozonated olive oil (OzOLO), any type, as single or adjuvant treatment | OzOLO (periodontitis trials; some gingivitis trials) and OLO (gingivitis trials) |
| Comparison | Conventional treatment, placebo, or no treatment | Chlorhexidine gel, scaling and root planing alone, or placebo/no treatment |
| Outcomes | Change in PI, BI, GI, PPD, CAL, BoP | PPD, CAL, BoP (periodontitis); PI, BI, GI (gingivitis) |
| Study designs | RCTs with at least 10 participants | 12 RCTs (parallel and split-mouth) |
| Timing | Baseline to one, three and six months | Periodontitis 3-4, 8-12 and 12-24 weeks; gingivitis 2-8 weeks |
AMSTAR 2 items
Two independent extractors rated each item. Their ratings are shown side by side because they have not been reconciled yet. The overall rating follows Shea et al. 2017: one critical flaw gives Low, more than one gives Critically Low.
| Item | Extractor A | Extractor B | Agree |
|---|---|---|---|
| 1Research question includes PICO components | Yes | Yes | yes |
| 2Protocol established before the reviewcritical | Partial Yes | Partial Yes | yes |
| 3Choice of study designs explained | No | No | yes |
| 4Comprehensive literature searchcritical | Partial Yes | Partial Yes | yes |
| 5Study selection in duplicate | Yes | Yes | yes |
| 6Data extraction in duplicate | Yes | Yes | yes |
| 7List of excluded studies with reasonscritical | Yes | Yes | yes |
| 8Included studies described in detail | Partial Yes | Partial Yes | yes |
| 9Risk of bias assessed satisfactorilycritical | RCT: Yes | NRSI: N/A · RCT: Yes | no |
| 10Funding of included studies reported | Yes | Yes | yes |
| 11Appropriate meta-analysis methodscritical | RCT: No | NRSI: N/A · RCT: No | no |
| 12Impact of risk of bias on the pooled results | No | No | yes |
| 13Risk of bias accounted for in interpretationcritical | No | No | yes |
| 14Heterogeneity explained | Yes | Yes | yes |
| 15Publication bias investigatedcritical | Yes | Yes | yes |
| 16Conflicts of interest reported | Yes | Yes | yes |
| Overall | Critically Low | Critically Low | yes |