Non-Cochrane · aetiology · PM35048071
Meta-Analysis Using NGS Data: The Veillonella Species in Dental Caries.
Dame-Teixeira Naile, de Lima Ana Karolina Almeida, Do Thuy, et al. · Frontiers in oral health · 2021 · PubMed · doi:10.3389/froh.2021.770917
Full textEvery question was extracted from the full text, figures and supplements by two independent LLM extractors, disagreements adjudicated. Rows cite where in the review the value comes from.
Questions
4
current version
GRADE rated
1
No studies
0
Included studies
38
linked below
No studies 0 · 0%Ungraded 3 · 75%Very low 1 · 25%Low 0 · 0%Moderate 0 · 0%High 0 · 0%
Ungraded means an effect was reported without a GRADE rating; it is not a gap. No studies means the review found nothing to answer the question. The six categories are a display order, not a score.
Review question
| Element | Planned (methods) | Found (results) |
|---|---|---|
| Population | Humans with dental caries, root caries, or early childhood caries (ECC) | 38 studies with 1,374 caries and 745 caries-free individuals (1,385 caries samples); ECC meta-analysis: 169 caries and 150 caries-free children (6 studies) |
| Intervention | Dental caries / root caries (exposure), at any presentation | Dental caries including ECC, coronal, root, and dentinal caries (n=38 studies; published 2014-2021) |
| Comparison | No dental caries (caries-free) or no control group | Caries-free controls (not all included studies had a control group) |
| Outcomes | Abundance of Veillonella at any taxonomic level (proportion, abundance, average) | Relative abundance of Veillonella spp., V. parvula, V. dispar, V. atypica by 16S rRNA amplicon (33 studies), shotgun metagenomics (3) and metatranscriptomics (2) |
| Study designs | Observational and clinical studies applying NGS methods | 38 observational/clinical NGS studies (24 sampled supragingival biofilm; saliva and carious dentin also sampled) |
| Timing | Not specified (no time restriction) | Not specified |
Search date: January 2021. Databases: MEDLINE (PubMed), LILACS, Web of Science, Scopus, Embase, Cochrane, Livivo; grey literature: Google Scholar, ProQuest, OpenGrey; plus reference lists of included studies
Questions and results
Dental caries (early childhood caries, ECC) vs Caries-free controls
Population: Children with early childhood caries (ECC) and caries-free children
| Outcome | Effect | Evidence | Status / GRADE |
|---|---|---|---|
Relative abundance of Veillonella spp. Overall (all sample types) Veillonella spp. is more abundant in children with early childhood caries than in caries-free children (mean difference 2.22, 95% CI 0.54 to 3.90); very low certainty. Source: Abstract; Results (Qualitative and Quantitative Synthesis); Figure 3 (overall diamond); Appendix Table 4 (GRADE Summary of Findings) · same question in other reviews | MD 2.22 [0.54, 3.90] | 6 studies · 319 participants | Very low Downgraded: risk of bias |
Relative abundance of Veillonella spp. Saliva In saliva, no significant difference in Veillonella spp. abundance was seen between ECC and caries-free children (mean difference -1.05, 95% CI -7.67 to 5.57); ungraded subgroup. Source: Figure 3 (forest plot, subgroup 1.1.2 Saliva); Results · same question in other reviews | MD -1.05 [-7.67, 5.57] | 2 studies · 161 participants | Ungraded |
Relative abundance of Veillonella spp. Supragingival biofilm/plaque In supragingival biofilm, Veillonella spp. was significantly enriched in ECC versus caries-free children (mean difference 3.11, 95% CI 0.67 to 5.55); ungraded subgroup. Source: Figure 3 (forest plot, subgroup 1.1.1 Supragingival Plaque); Results · same question in other reviews | MD 3.11 [0.67, 5.55] | 4 studies · 158 participants | Ungraded |
Comparison
| Outcome | Effect | Evidence | Status / GRADE |
|---|---|---|---|
Relative abundance of Veillonella spp. Sensitivity analysis excluding 2 low-quality studies [12,17] | MD 2.07 [-2.44, 6.58] | 4 studies | Ungraded |
Included primary studies 38
Open as a trial list| Study | Also included by | Outcomes it informs here | Trial PICO | Results |
|---|---|---|---|---|
| Dashper 2019 Temporal development of the oral microbiome and prediction of early childhood caries. PMID:31874981 | 1 other review | not stated | not extracted yet | not extracted yet |
| de Jesus 2020 Sex-Based Diverse Plaque Microbiota in Children with Severe Caries DOI:10.1177/0022034520908595 | 1 other review | Relative abundance of Veillonella spp. | not extracted yet | not extracted yet |
| Eriksson 2017 Saliva and tooth biofilm bacterial microbiota in adolescents in a low caries community. PMID:28724921 | 1 other review | not stated | not extracted yet | not extracted yet |
| Zheng 2018 Comparative Analysis of the Microbial Profiles in Supragingival Plaque Samples Obtained From Twins With Discordant Caries Phenotypes and… PMID:30460203 | 1 other review | not stated | not extracted yet | not extracted yet |
| Agnello 2017 Microbiome Associated with Severe Caries in Canadian First Nations Children. PMID:28709393 | this review only | not stated | not extracted yet | not extracted yet |
| Al-Hebshi 2019 Metagenome sequencing-based strain-level and functional characterization of supragingival microbiome associated with dental caries in… PMID:30671194 | this review only | Relative abundance of Veillonella spp. | not extracted yet | not extracted yet |
| Alcaraz 2012 Identifying a healthy oral microbiome through metagenomics DOI:10.1111/j.1469-0691.2012.03857.x | this review only | not stated | not extracted yet | not extracted yet |
| Belda-Ferre 2012 The oral metagenome in health and disease. PMID:21716308 | this review only | not stated | not extracted yet | not extracted yet |
| Belstrom 2017 Salivary microbiota in individuals with different levels of caries experience. PMID:28326153 | this review only | not stated | not extracted yet | not extracted yet |
| Chen 2020 The oral microbiome and salivary proteins influence caries in children aged 6 to 8 years. PMID:33115458 | this review only | not stated | not extracted yet | not extracted yet |
| Corralo 2018 matched by author and year (probable) | this review only | not stated | not extracted yet | not extracted yet |
| Dame-Teixeira 2020 Presence of Archaea in dental caries biofilms DOI:10.1016/j.archoralbio.2019.104606 | this review only | not stated | not extracted yet | not extracted yet |
| Do 2015 Transcriptomic analysis of three Veillonella spp. present in carious dentine and in the saliva of caries-free individuals. PMID:25859434 | this review only | not stated | not extracted yet | not extracted yet |
| Foxman 2016 The effects of family, dentition, and dental caries on the salivary microbiome. PMID:27157862 | this review only | not stated | not extracted yet | not extracted yet |
| Grier 2020 Oral Microbiota Composition Predicts Early Childhood Caries Onset. PMID:33356775 | this review only | not stated | not extracted yet | not extracted yet |
| Hurley 2019 Comparison of the salivary and dentinal microbiome of children with severe-early childhood caries to the salivary microbiome of… PMID:30642327 | this review only | Relative abundance of Veillonella spp. | not extracted yet | not extracted yet |
| Jagathrakshakan 2015 16S rRNA gene-based metagenomic analysis identifies a novel bacterial co-prevalence pattern in dental caries. PMID:25713496 | this review only | not stated | not extracted yet | not extracted yet |
| Jiang 2019 The Oral Microbiome in the Elderly With Dental Caries and Health. PMID:30662876 | this review only | not stated | not extracted yet | not extracted yet |
| Meng 2015 Pyrosequencing of Plaque Microflora In Twin Children with Discordant Caries Phenotypes. PMID:26524687 | this review only | not stated | not extracted yet | not extracted yet |
| Mitwalli 2019 Effect of Silver Diamine Fluoride Treatment on Microbial Profiles of Plaque Biofilms from Root/Cervical Caries Lesions DOI:10.1159/000499578 | this review only | not stated | not extracted yet | not extracted yet |
| Nomura 2020 Oral Microbiome of Children Living in an Isolated Area in Myanmar. PMID:32517039 | this review only | not stated | not extracted yet | not extracted yet |
| Ortiz 2019 Sex-specific differences in the salivary microbiome of caries-active children. PMID:31497256 | this review only | not stated | not extracted yet | not extracted yet |
| Qudeimat 2021 Dental plaque microbiota profiles of children with caries-free and caries-active dentition DOI:10.1016/j.jdent.2020.103539 | this review only | not stated | not extracted yet | not extracted yet |
| Ribeiro 2017 The oral bacterial microbiome of occlusal surfaces in children and its association with diet and caries. PMID:28678838 | this review only | not stated | not extracted yet | not extracted yet |
| Richards 2017 Microbiomes of Site-Specific Dental Plaques from Children with Different Caries Status. PMID:28507066 | this review only | not stated | not extracted yet | not extracted yet |
| Rocas 2016 Microbiome of Deep Dentinal Caries Lesions in Teeth with Symptomatic Irreversible Pulpitis. PMID:27135405 | this review only | not stated | not extracted yet | not extracted yet |
| Schulze-Schweifing 2012 matched by author and year (probable) | this review only | not stated | not extracted yet | not extracted yet |
| Simon-Soro 2013 A Tissue-Dependent Hypothesis of Dental Caries DOI:10.1159/000351663 | this review only | not stated | not extracted yet | not extracted yet |
| Simon-Soro 2014 Metatranscriptomics reveals overall active bacterial composition in caries lesions. PMID:25626770 | this review only | not stated | not extracted yet | not extracted yet |
| Tian 2015 Microbiome interaction with sugar plays an important role in relapse of childhood caries DOI:10.1016/j.bbrc.2015.10.110 | this review only | not stated | not extracted yet | not extracted yet |
| Wolff 2019 Amplicon-based microbiome study highlights the loss of diversity and the establishment of a set of species in patients with dentin caries. PMID:31365560 | this review only | not stated | not extracted yet | not extracted yet |
| Xiao 2016 Bacterial Diversity and Community Structure of Supragingival Plaques in Adults with Dental Health or Caries Revealed by 16S Pyrosequencing. PMID:27499752 | this review only | not stated | not extracted yet | not extracted yet |
| Xiao 2018 Association between Oral Candida and Bacteriome in Children with Severe ECC. PMID:30049240 | this review only | not stated | not extracted yet | not extracted yet |
| Xu and Chen 2018 Dynamic Alterations in Salivary Microbiota Related to Dental Caries and Age in Preschool Children With Deciduous Dentition: A 2-Year… PMID:29670544 | this review only | Relative abundance of Veillonella spp. | not extracted yet | not extracted yet |
| Xu and Hao 2014 Plaque bacterial microbiome diversity in children younger than 30 months with or without caries prior to eruption of second primary molars. PMID:24586647 | this review only | Relative abundance of Veillonella spp. | not extracted yet | not extracted yet |
| Xu and Jia 2018 Metagenomic analysis of oral microbiome in young children aged 6–8 years living in a rural isolated Chinese province DOI:10.1111/odi.12871 | this review only | not stated | not extracted yet | not extracted yet |
| Xu and Tian 2018 Oral Microbiome Shifts From Caries-Free to Caries-Affected Status in 3-Year-Old Chinese Children: A Longitudinal Study. PMID:30210479 | this review only | Relative abundance of Veillonella spp. | not extracted yet | not extracted yet |
| Yun 2019 Illumina-based sequencing analysis of pathogenic microorganisms in dental caries patients of different Chinese ethnic groups. PMID:31516041 | this review only | not stated | not extracted yet | not extracted yet |
Overlap with other reviews
Reviews that include the same primary studies are not independent evidence. Shared counts match studies by PMID, DOI or NCT, and otherwise by author and year (a probable match).