Non-Cochrane · intervention · PM42004803
In vitro impact of storage media on extracted teeth: Systematic review and meta-analysis of adhesive, mechanical, and crack-related outcomes.
Keswani Kiran, Aggarwal Shalini · Journal of conservative dentistry and endodontics · 2026 · PubMed · doi:10.4103/jcde.jcde_7_26
Findings
5 findings
| Population | ||||
|---|---|---|---|---|
| Extracted human teeth | Less favorable storage condition (chloramine at 4C for 48 days or longer; formalin) | Fresh teeth or chloramine storage | Microleakage (dye penetration) | MD 0.97 [0.59, 1.35] GRADE not reported 2 studies |
| Extracted human teeth | Storage in sodium hypochlorite (NaOCl) | Storage in distilled water (DW) | Fracture resistance (load to fracture, N) 14, 90 and 180 days (time-matched contrasts pooled) | MD -84.5 [-241.3, 72.2] GRADE not reported 1 study · 60 participants |
| Extracted human teeth (intact or restored) tested in vitro | Sodium hypochlorite (NaOCl) storage | Distilled water (DW) storage | Fracture resistance (maximum load to failure) 180 days · Laboratory (extracted human teeth) · 180-day interval only (sensitivity analysis) | MD -84.1 [-315.7, 147.5] GRADE not reported 1 study |
| Extracted human teeth | Cryostorage at -80C | Cryostorage at -196C | Fracture resistance (load to fracture, N) 2 weeks | MD -310.0 [-743.6, 123.6] GRADE not reported 1 study · 20 participants |
| Extracted human teeth | Dry or air-dry storage (dehydration) | Moist or wet storage | Artifacts or microcracks on imaging or microscopy (at least one defect) | OR 56.66 [9.77, 328.75] GRADE not reported 3 studies · 110 participants |
Included primary studies 27
Open in Studies| Study | Year | Also included by | Outcomes it informs here | Trial data |
|---|---|---|---|---|
| Angermair 2020 The influence of storage temperature on fracture behavior of cryopreserved teeth—An in vitro study DOI:10.1002/cre2.283 | 2020 | this review only | Fracture resistance (load to fracture, N) | not extracted yet |
| Angermair 2020 The influence of storage temperature on fracture behavior of cryopreserved teeth-An in vitro study. PMID:32222040 | 2020 | this review only | not stated | not extracted yet |
| Arashiro 2019 matched by author and year (probable) | NR | this review only | Microleakage (dye penetration); Artifacts or microcracks on imaging or microscopy (at least one defect) | not extracted yet |
| Camps 1996 Influence of tooth cryopreservation and storage time on microleakage DOI:10.1016/s0109-5641(96)80079-0 | 1996 | this review only | Microleakage (dye penetration) | not extracted yet |
| Camps 1996 Influence of tooth cryopreservation and storage time on microleakage. PMID:9002854 | 1996 | this review only | Fracture resistance (load to fracture, N) | not extracted yet |
| Da Silva 2015 Effect of Chlorhexidine on Dentin Bond Strength of Two Adhesive Systems after Storage in Different Media DOI:10.1590/0103-6440201300159 | 2015 | this review only | not stated | not extracted yet |
| Da Silva 2015 Effect of Chlorhexidine on Dentin Bond Strength of Two Adhesive Systems after Storage in Different Media. PMID:26963210 | 2015 | this review only | not stated | not extracted yet |
| De-Deus 2024 The impact of long‐term storage conditions on the development of experimental dentinal microcracks DOI:10.1111/aej.12806 | 2023 | this review only | Artifacts or microcracks on imaging or microscopy (at least one defect) | not extracted yet |
| De-Deus 2024 The impact of long-term storage conditions on the development of experimental dentinal microcracks. PMID:37876340 | 2024 | this review only | Microleakage (dye penetration) | not extracted yet |
| Goodis 1993 Storage effects on dentin permeability and shear bond strengths DOI:10.1016/0109-5641(93)90079-6 | 1993 | this review only | not stated | not extracted yet |
| Goodis 1993 Storage effects on dentin permeability and shear bond strengths. PMID:8595846 | 1993 | this review only | not stated | not extracted yet |
| Haller 1993 Effect of storage media on microleakage of five dentin bonding agents DOI:10.1016/0109-5641(93)90119-b | 1993 | this review only | Microleakage (dye penetration) | not extracted yet |
| Haller 1993 Effect of storage media on microleakage of five dentin bonding agents. PMID:8056175 | 1993 | this review only | Fracture resistance (load to fracture, N) | not extracted yet |
| Hashim 2022 matched by author and year (probable) | NR | this review only | Fracture resistance (load to fracture, N) | not extracted yet |
| Hashim 2022 Measurement of radii of curvature of convex and concave surfaces using a nodal bench and He-Ne laser. PMID:20221116 | 1980 | this review only | Fracture resistance (load to fracture, N) | not extracted yet |
| Jarahi 2018 matched by author and year (probable) | NR | this review only | not stated | not extracted yet |
| Jarahi 2018 Mutational and immunohistochemical study of the PI3K/Akt pathway in papillary thyroid carcinoma in Greece. PMID:20186503 | 2010 | this review only | not stated | not extracted yet |
| Mobarak 2010 Effect of pretest storage conditions of extracted teeth on their dentin bond strengths DOI:10.1016/s0022-3913(10)60098-4 | 2010 | this review only | not stated | not extracted yet |
| Mobarak 2010 Effect of pretest storage conditions of extracted teeth on their dentin bond strengths. PMID:20654765 | 2010 | this review only | not stated | not extracted yet |
| Panighi 1997 Influence of human tooth cryopreservation on dentin bond strength DOI:10.1016/s0109-5641(97)80009-7 | 1997 | this review only | not stated | not extracted yet |
| Panighi 1997 Influence of human tooth cryopreservation on dentin bond strength. PMID:9467324 | 1997 | this review only | not stated | not extracted yet |
| Shemesh 2018 Dehydration Induces Cracking in Root Dentin Irrespective of Instrumentation: A Two-dimensional and Three-dimensional Study DOI:10.1016/j.joen.2017.07.025 | 2018 | this review only | Artifacts or microcracks on imaging or microscopy (at least one defect) | not extracted yet |
| Shemesh 2018 Dehydration Induces Cracking in Root Dentin Irrespective of Instrumentation: A Two-dimensional and Three-dimensional Study. PMID:29079053 | 2018 | this review only | Microleakage (dye penetration) | not extracted yet |
| Toledano 2007 Durability of resin–dentin bonds: Effects of direct/indirect exposure and storage media DOI:10.1016/j.dental.2006.06.030 | 2007 | this review only | not stated | not extracted yet |
| Toledano 2007 Durability of resin-dentin bonds: effects of direct/indirect exposure and storage media. PMID:16949659 | 2007 | this review only | not stated | not extracted yet |
| Yilmaz 2023 Effects of Different Storage Media on Dentin Moisture, Microhardness, and Bond Strength of Resin Composite DOI:10.4103/njcp.njcp_498_22 | 2023 | this review only | not stated | not extracted yet |
| Yilmaz 2023 Effects of different storage media on dentin moisture, microhardness, and bond strength of resin composite. PMID:36876610 | 2023 | this review only | not stated | not extracted yet |
Review question
| Element | Planned (methods) | Found (results) |
|---|---|---|
| Population | In vitro or ex vivo studies on extracted human teeth (intact or restored) | Extracted human teeth (n=14 studies) |
| Intervention | Any storage or handling condition (liquid media, dry storage, temperature manipulation, preimaging dehydration) | Dry/air-dry storage; chloramine, formalin, thymol, ethanol, NaOCl, distilled water, mineral oil; cryopreservation; autoclaving |
| Comparison | Another storage or handling condition or a concurrent control group | Moist/wet storage; fresh teeth; distilled water; other media; -196 C versus -80 C |
| Outcomes | Fracture resistance; bond strength; microleakage; artifacts or microcracks on imaging | Artifacts (3 studies pooled); microleakage (2 studies pooled); fracture resistance (1 study pooled over time points); bond strength (not pooled) |
| Study designs | Laboratory (in vitro or ex vivo) comparative studies with parallel or matched groups | In vitro experimental studies (n=14) |
| Timing | Not specified | Storage from days to about 2 years |
AMSTAR 2 items
Two independent extractors rated each item. Their ratings are shown side by side because they have not been reconciled yet. The overall rating follows Shea et al. 2017: one critical flaw gives Low, more than one gives Critically Low.
| Item | Extractor A | Extractor B | Agree |
|---|---|---|---|
| 1Research question includes PICO components | Yes | Yes | yes |
| 2Protocol established before the reviewcritical | Partial Yes | Partial Yes | yes |
| 3Choice of study designs explained | No | No | yes |
| 4Comprehensive literature searchcritical | Partial Yes | Partial Yes | yes |
| 5Study selection in duplicate | Yes | Yes | yes |
| 6Data extraction in duplicate | Yes | Yes | yes |
| 7List of excluded studies with reasonscritical | No | No | yes |
| 8Included studies described in detail | Partial Yes | Partial Yes | yes |
| 9Risk of bias assessed satisfactorilycritical | RCT: N/A · NRSI: Yes | NRSI: Partial Yes · RCT: Partial Yes | no |
| 10Funding of included studies reported | No | No | yes |
| 11Appropriate meta-analysis methodscritical | RCT: N/A · NRSI: No | NRSI: No · RCT: No | no |
| 12Impact of risk of bias on the pooled results | No | No | yes |
| 13Risk of bias accounted for in interpretationcritical | Yes | Yes | yes |
| 14Heterogeneity explained | Yes | Yes | yes |
| 15Publication bias investigatedcritical | No | No | yes |
| 16Conflicts of interest reported | Yes | Yes | yes |
| Overall | Critically Low | Critically Low | yes |