Non-Cochrane · aetiology · PM41660898
Micronuclei in the Buccal Mucosal Cells are Genotoxicity Markers in Oral Potentially Malignant Disorders: A Systematic Review and Meta-Analysis.
Chauhan Anoushka, Angadi Punnya V · Asian Pacific journal of cancer prevention : APJCP · 2026 · PubMed · doi:10.31557/apjcp.2026.27.2.413
Findings
5 findings
| Population | ||||
|---|---|---|---|---|
| People with oral potentially malignant disorders (OPMD, unsegregated) and healthy controls | oral potentially malignant disorders (OPMD, unsegregated) | Healthy controls | Micronuclei (MN) count in exfoliated buccal mucosal cells Overall estimate as stated in Abstract | SMD 3.08 [1.80, 4.35] GRADE not reported Studies not reported |
| People with oral potentially malignant disorders (OPMD, unsegregated) and healthy controls | oral potentially malignant disorders (OPMD, unsegregated) | Healthy controls | Micronuclei (MN) count in exfoliated buccal mucosal cells | SMD 2.14 [0.13, 4.15] GRADE not reported 9 studies · 925 participants |
| People with oral leukoplakia and healthy controls | oral leukoplakia | Healthy controls | Micronuclei (MN) count in exfoliated buccal mucosal cells | SMD 2.75 [1.76, 3.74] GRADE not reported 11 studies · 738 participants |
| People with oral lichen planus and healthy controls | oral lichen planus | Healthy controls | Micronuclei (MN) count in exfoliated buccal mucosal cells | SMD 1.47 [1.12, 1.82] GRADE not reported 6 studies · 349 participants |
| People with oral submucous fibrosis (OSMF) and healthy controls | oral submucous fibrosis (OSMF) | Healthy controls | Micronuclei (MN) count in exfoliated buccal mucosal cells | SMD 5.55 [0.77, 10.32] GRADE not reported 11 studies · 889 participants |
Included primary studies 28
Open in Studies| Study | Year | Also included by | Outcomes it informs here | Trial data |
|---|---|---|---|---|
| Bhatnagar 2023 matched by author and year (probable) | NR | this review only | not stated | not extracted yet |
| Dave 2019 Evaluation of Micronucleus Frequencies in Exfoliated Buccal Cells of Potentially Malignant Disorder Subjects Using Feulgen Stain DOI:10.5866/2019.11.10086 | 2019 | this review only | not stated | not extracted yet |
| Grover 2014 matched by author and year (probable) | NR | this review only | not stated | not extracted yet |
| Grover 2014 Ruthenium intermetallics grown from La-Ni flux: synthesis, structure, and physical properties. PMID:20141115 | 2010 | this review only | not stated | not extracted yet |
| Gupta 2019 Comparison of different stains in exfoliated oral mucosal cell micronucleus of potentially malignant disorders of oral cavity. PMID:31169229 | 2019 | this review only | not stated | not extracted yet |
| Jaiswal and Sharma 2019 An assessment of Genotoxic Damage in Exfoliated Buccal Cells using Saliva as a tool DOI:10.4103/jasi.jasi_99_19 | 2019 | this review only | not stated | not extracted yet |
| Joshi 2010 Cytogenetic alterations in buccal mucosa cells of chewers of areca nut and tobacco. PMID:20863483 | 2011 | this review only | not stated | not extracted yet |
| Juneja 2019 Utility of DNA-Specific Stains in Micronuclei Assay as a Marker of Genotoxicity in Oral Potentially Malignant Disorders and Oral Squamous… PMID:30992647 | 2019 | this review only | not stated | not extracted yet |
| Jyoti 2012 Micronucleus investigation in human buccal epithelial cells of gutkha users. PMID:23326766 | 2012 | this review only | not stated | not extracted yet |
| Kamboj 2006 Micronucleus--an upcoming marker of genotoxic damage. PMID:17031674 | 2007 | this review only | not stated | not extracted yet |
| Katarkar 2014 Comparative evaluation of genotoxicity by micronucleus assay in the buccal mucosa over comet assay in peripheral blood in oral precancer… PMID:25053835 | 2014 | this review only | not stated | not extracted yet |
| Khalida Begum 2016 Unblocking brainstorms. PMID:2016214 | 1991 | this review only | not stated | not extracted yet |
| Khanna 2014 Cytogenetic biomonitoring of premalignant and malignant oral lesions by micronuclei assessment: A screening evaluation DOI:10.4103/2278-9626.126211 | 2014 | this review only | not stated | not extracted yet |
| Kohli 2017 Micronucleus Assay: An Early Diagnostic Tool to Assess Genotoxic Changes in Patients with Tobacco Use, Oral Leukoplakia and Oral Submucous… PMID:29207828 | 2017 | this review only | not stated | not extracted yet |
| Koneru 2011 Comparative study of oral micronucleated cell frequency in oral submucous fibrosis patients and healthy individuals DOI:10.4317/jced.3.e201 | 2011 | this review only | not stated | not extracted yet |
| Mahimkar 2010 Influence of genetic polymorphisms on frequency of micronucleated buccal epithelial cells in leukoplakia patients. PMID:20850372 | 2010 | this review only | not stated | not extracted yet |
| Neha Gupta 2019 Comparative evaluation of micronuclei in exfoliated oral epithelial cells in potentially malignant disorders and malignant lesions using… PMID:31110437 | 2019 | this review only | not stated | not extracted yet |
| Paravathi Devi 2011 Micronucleus Assay for Evaluation of Genotoxicity in Potentially Malignant and Malignant Disorders DOI:10.5005/jp-journals-10011-1103 | 2011 | this review only | not stated | not extracted yet |
| Pushapanjali 2017 matched by author and year (probable) | NR | this review only | not stated | not extracted yet |
| Pushpanjali 2017 matched by author and year (probable) | NR | this review only | not stated | not extracted yet |
| Sanchez-Siles 2011 A novel application of the buccal micronucleus cytome assay in oral lichen planus: a pilot study. PMID:21429474 | 2011 | this review only | not stated | not extracted yet |
| Sangle 2016 Comparative study of frequency of micronuclei in normal, potentially malignant diseases and oral squamous cell carcinoma. PMID:27003966 | 2016 | this review only | not stated | not extracted yet |
| Sarahanglu 2013 Evaluation of micronuclear frequencies in both circulating lymphocytes and buccal epithelial cells of patients with oral lichen planus and… PMID:24033885 | 2014 | this review only | not stated | not extracted yet |
| Saran 2007 Risk assessment of oral cancer in patients with pre-cancerous states of the oral cavity using micronucleus test and challenge assay. PMID:17936677 | 2008 | this review only | not stated | not extracted yet |
| Singam 2019 Evaluation of genotoxicity by micronucleus assay in oral leukoplakia and oral squamous cell carcinoma with deleterious habits. PMID:31516241 | 2019 | this review only | not stated | not extracted yet |
| Sudha 2023 Salivary Oxidative Stress Assessment in Tobacco Users with and without Potentially Malignant Disorders and Micronuclei Estimation using… PMID:42040215 | 2026 | this review only | not stated | not extracted yet |
| Vidyalakshmi 2016 Buccal Micronuclei Assay as a Tool For Biomonitoring DNA Damage in Oral Lichen Planus. PMID:27630941 | 2016 | this review only | not stated | not extracted yet |
| Wagh 2018 Micronuclei in Exfoliated Oral Epithelial Cells in Tobacco Users and Controls with Various Oral Lesions: A Study from Gujarat, India. PMID:30906725 | 2019 | this review only | not stated | not extracted yet |
Review question
| Element | Planned (methods) | Found (results) |
|---|---|---|
| Population | Human subjects with oral potentially malignant disorders (leukoplakia, oral lichen planus, oral submucous fibrosis) with control groups | 1,489 OPMD cases (417 leukoplakia, 180 oral lichen planus, 401 OSMF, 491 unsegregated OPMDs) and 1,078 healthy controls (26 studies; 25 in meta-analysis) |
| Intervention | Oral potentially malignant disorders (micronuclei assessed in buccal cells) | OPMD: leukoplakia (11 studies in meta-analysis), oral lichen planus (6), OSMF (11), unsegregated OPMD (9) |
| Comparison | Healthy controls | Healthy controls |
| Outcomes | Micronuclei frequency (mean count) in exfoliated buccal mucosal cells as a genotoxicity marker | Mean micronuclei count in buccal cells (Cohen's d) |
| Study designs | Peer-reviewed original studies in English with control groups (observational) | Observational studies, mostly Indian population; modified Newcastle-Ottawa Scale |
| Timing | Not specified | Not specified |