Findings

What do systematic reviews say?

7 questions · 7 findings · 1 reviews · Dental caries

No studies found 0 · 0%GRADE not reported 3 · 42.9%Very low 4 · 57.1%Low 0 · 0%Moderate 0 · 0%High 0 · 0%

MMP-2 activity · Human root and coronal carious dentin

No difference in MMP-2 activity was found in root versus coronal carious dentin (very low certainty).

1 finding from 1 review

MMP-2 presence/activity · Human ex vivo carious dentin

MMP-2 shows no difference between root and coronal carious dentin; the evidence is very uncertain (very low certainty).

1 finding from 1 review

Presence of host and bacterial-derived collagenolytic proteases in carious dentin: a systematic review of ex vivo… 2023 All outcomes
Ex vivo (extracted teeth / clinical carious or biofilm samples)
There is no difference in MMP-2 activity in root vs. coronal carious dentin; MMP-2 was present in both coronal and root dentin in all specimens (Toledano et al., 2010). The evidence is very uncertain.
1 study · 10 participants
Very low

MMP-13 presence/activity · Human ex vivo carious dentin

MMP-13 may be increased in root compared with coronal carious dentin, but the evidence is very uncertain (very low certainty).

1 finding from 1 review

Presence of host and bacterial-derived collagenolytic proteases in carious dentin: a systematic review of ex vivo… 2023 All outcomes
Ex vivo (extracted teeth / clinical carious or biofilm samples)
MMP-13 may be increased in root caries when compared to coronal, but the evidence is very uncertain; no MMP-13 activity observed in the crown region by western blot (Yeon Lee et al., 2013).
1 study · 7 participants
Very low

MMP-13 activity · Human root and coronal carious dentin

MMP-13 may be increased in root compared with coronal carious dentin (very low certainty).

1 finding from 1 review

Collagen-degrading bacteria · Root caries lesion biofilm

Protein-degrading bacteria capable of degrading collagen were detected in root caries biofilm.

1 finding from 1 review

Bacterial collagenolytic protease gene expression · Human ex vivo carious lesions / caries-associated biofilm

Genes encoding bacterial collagenolytic proteases were detected/overexpressed in coronal and root caries (ungraded; GRADE not feasible).

1 finding from 1 review

Presence of host and bacterial-derived collagenolytic proteases in carious dentin: a systematic review of ex vivo… 2023 All outcomes
Ex vivo (extracted teeth / clinical carious or biofilm samples)
Genes coding for bacterial collagenolytic proteases were significantly expressed/overrepresented in coronal (Simon-Soro et al., 2013) and root (Dame-Teixeira et al., 2018: 42 proteases differentially expressed, 18…
2 studies · 25 participants
GRADE not reported

Bacterial collagenolytic enzymatic activity · Human ex vivo carious lesions / caries-associated biofilm

Bacterial collagenolytic enzymatic activity in caries biofilm is inconclusive across two small studies (ungraded; GRADE not feasible).

1 finding from 1 review

Presence of host and bacterial-derived collagenolytic proteases in carious dentin: a systematic review of ex vivo… 2023 All outcomes
Ex vivo (extracted teeth / clinical carious or biofilm samples)
Results are inconclusive: protein-degrading bacteria isolated from root caries biofilm were capable of degrading collagen (Prevotella, Actinobaculum, Propionibacterium predominant; proportion 7% in root caries)…
2 studies · 9 participants
GRADE not reported