Non-Cochrane · intervention · PM41622190
Effects of mussel-inspired compounds on dentin bonding: a systematic review and meta-analysis.
Peng Fangfei, Long Yu, Gao Huilin, et al. · BMC oral health · 2026 · PubMed · doi:10.1186/s12903-026-07802-z
Findings
13 findings
| Population | ||||
|---|---|---|---|---|
| Sound human or bovine dentin | Mussel-inspired compounds (monomeric and polymeric) applied in dentin bonding | Control without mussel-inspired compounds | Immediate resin-dentin bond strength (MTBS or SBS) Immediate · In vitro laboratory | SMD 0.91 [0.38, 1.44] GRADE not reported 11 studies · 756 participants |
| Sound human or bovine dentin | Mussel-inspired compounds (monomeric and polymeric) applied in dentin bonding | Control without mussel-inspired compounds | Reduction in bond strength after aging (difference between immediate and aged bond strength) After aging (thermocycling, in some studies with collagenase challenge) · In vitro laboratory · Sensitivity analysis excluding studies with positive controls | SMD -4.69 [-6.89, -2.49] GRADE not reported 7 studies · 540 participants |
| Sound human or bovine dentin | Mussel-inspired compounds (monomeric and polymeric) applied in dentin bonding | Control without mussel-inspired compounds | Immediate resin-dentin bond strength (MTBS or SBS) Immediate · In vitro laboratory · Sensitivity analysis excluding studies with positive controls | SMD 0.84 [0.27, 1.42] GRADE not reported 9 studies · 700 participants |
| Sound human or bovine dentin | Mussel-inspired compounds (monomeric and polymeric) applied in dentin bonding | Control without mussel-inspired compounds | Hydroxyproline (HYP) release after collagenase challenge After collagenase degradation · In vitro laboratory | SMD -9.04 [-12.86, -5.21] GRADE not reported 4 studies · 80 participants |
| Sound human or bovine dentin | Mussel-inspired compounds (monomeric and polymeric) applied in dentin bonding | Control without mussel-inspired compounds | Reduction in bond strength after aging (difference between immediate and aged bond strength) After aging (thermocycling, in some studies with collagenase challenge) · In vitro laboratory | SMD -3.97 [-6.01, -1.93] GRADE not reported 8 studies · 576 participants |
| Sound human or bovine dentin | Monomeric mussel-inspired compounds (DMA, CLM, OX-DAA, L-DOPA) applied in dentin bonding | Control without mussel-inspired compounds | Immediate resin-dentin bond strength (MTBS or SBS) Immediate · In vitro laboratory · Subgroup: monomeric form; sensitivity analysis excluding studies with positive controls | SMD 1.23 [0.73, 1.72] GRADE not reported 7 studies · 520 participants |
| Sound human or bovine dentin | Monomeric mussel-inspired compounds (DMA, CLM, OX-DAA, L-DOPA) applied in dentin bonding | Control without mussel-inspired compounds | Immediate resin-dentin bond strength (MTBS or SBS) Immediate · In vitro laboratory · Subgroup: monomeric form | SMD 1.26 [0.78, 1.73] GRADE not reported 8 studies · 540 participants |
| Sound human or bovine dentin | Monomeric mussel-inspired compounds (DMA, CLM, OX-DAA, L-DOPA) applied in dentin bonding | Control without mussel-inspired compounds | Reduction in bond strength after aging (difference between immediate and aged bond strength) After aging (thermocycling, in some studies with collagenase challenge) · In vitro laboratory · Subgroup: monomeric form | SMD -4.0 [-5.89, -2.1] GRADE not reported 6 studies · 400 participants |
| Sound human or bovine dentin | Monomeric mussel-inspired compounds (DMA, CLM, OX-DAA, L-DOPA) applied in dentin bonding | Control without mussel-inspired compounds | Reduction in bond strength after aging (difference between immediate and aged bond strength) After aging (thermocycling, in some studies with collagenase challenge) · In vitro laboratory · Subgroup: monomeric form; sensitivity analysis excluding studies with positive controls | SMD -4.0 [-5.89, -2.1] GRADE not reported 6 studies · 400 participants |
| Sound human or bovine dentin | Polymeric mussel-inspired compounds (pDMA-MEA, PDA, MAP) applied in dentin bonding | Control without mussel-inspired compounds | Reduction in bond strength after aging (difference between immediate and aged bond strength) After aging (thermocycling, in some studies with collagenase challenge) · In vitro laboratory · Subgroup: polymeric form | SMD -3.64 [-13.54, 6.27] GRADE not reported 2 studies · 176 participants |
| Sound human or bovine teeth (resin-dentin interface, in vitro) | Polymeric mussel-inspired compounds applied in dentin bonding | Control without mussel-inspired compounds | Reduction in resin-dentin bond strength after aging (immediate minus aged) after aging (thermocycling, in one study plus collagenase challenge) · In vitro (laboratory) · Polymeric form; Sensitivity analysis excluding studies with positive controls | SMD -8.7 [-9.78, -7.61] GRADE not reported 1 study · 140 participants |
| Sound human or bovine dentin | Polymeric mussel-inspired compounds (pDMA-MEA, PDA, MAP) applied in dentin bonding | Control without mussel-inspired compounds | Immediate resin-dentin bond strength (MTBS or SBS) Immediate · In vitro laboratory · Subgroup: polymeric form; sensitivity analysis excluding studies with positive controls | SMD -2.52 [-7.57, 2.53] GRADE not reported 2 studies · 180 participants |
| Sound human or bovine dentin | Polymeric mussel-inspired compounds (pDMA-MEA, PDA, MAP) applied in dentin bonding | Control without mussel-inspired compounds | Immediate resin-dentin bond strength (MTBS or SBS) Immediate · In vitro laboratory · Subgroup: polymeric form | SMD -1.21 [-3.56, 1.14] GRADE not reported 3 studies · 216 participants |
Included primary studies 23
Open in Studies| Study | Year | Also included by | Outcomes it informs here | Trial data |
|---|---|---|---|---|
| Fang 2017 Anti-proteolytic property and bonding durability of mussel adhesive protein-modified dentin adhesive interface DOI:10.1016/j.dental.2017.07.008 | 2017 | this review only | not stated | not extracted yet |
| Fang 2017 Anti-proteolytic property and bonding durability of mussel adhesive protein-modified dentin adhesive interface. PMID:28754254 | 2017 | this review only | not stated | not extracted yet |
| Hu 2022 Mussel-inspired polymer with catechol and cationic Lys functionalities for dentin wet bonding DOI:10.1016/j.mtbio.2022.100506 | 2023 | this review only | not stated | not extracted yet |
| Hu 2022 Mussel-inspired polymer with catechol and cationic Lys functionalities for dentin wet bonding. PMID:36471892 | 2023 | this review only | not stated | not extracted yet |
| Lee 2015 Catechol-Functionalized Synthetic Polymer as a Dental Adhesive to Contaminated Dentin Surface for a Composite Restoration DOI:10.1021/acs.biomac.5b00451 | 2015 | this review only | not stated | not extracted yet |
| Lee 2015 Catechol-Functionalized Synthetic Polymer as a Dental Adhesive to Contaminated Dentin Surface for a Composite Restoration. PMID:26176305 | 2015 | this review only | not stated | not extracted yet |
| Li 2020 The application of mussel-inspired molecule in dentin bonding DOI:10.1016/j.jdent.2020.103404 | 2020 | this review only | not stated | not extracted yet |
| Li 2020 The application of mussel-inspired molecule in dentin bonding. PMID:32522689 | 2020 | this review only | not stated | not extracted yet |
| Li 2021 Mussel wet adhesion inspired using dopamine methacrylamide as a primer to improve dentin adhesion DOI:10.1080/01694243.2021.1888580 | 2021 | this review only | not stated | not extracted yet |
| Li 2021 (J Dent, HYP) In vitro evaluation of the anti-proteolytic and cross-linking effect of mussel-inspired monomer on the demineralized dentin matrix. PMID:34119610 | 2021 | this review only | not stated | not extracted yet |
| Li 2021 (J Dent) In vitro evaluation of the anti-proteolytic and cross-linking effect of mussel-inspired monomer on the demineralized dentin matrix DOI:10.1016/j.jdent.2021.103720 | 2021 | this review only | not stated | not extracted yet |
| Li 2021 (Mater Today Bio) Enhancing resin-dentin bond durability using a novel mussel-inspired monomer DOI:10.1016/j.mtbio.2021.100174 | 2021 | this review only | not stated | not extracted yet |
| Li 2021 (Mater Today Bio) Enhancing resin-dentin bond durability using a novel mussel-inspired monomer. PMID:34901824 | 2021 | this review only | not stated | not extracted yet |
| Li 2022 Mussel-inspired monomer – A new selective protease inhibitor against dentine collagen degradation DOI:10.1016/j.dental.2022.05.002 | 2022 | this review only | not stated | not extracted yet |
| Li 2022 Mussel-inspired monomer - A new selective protease inhibitor against dentine collagen degradation. PMID:35680429 | 2022 | this review only | not stated | not extracted yet |
| Pereira 2024 Properties of a Dental Adhesive Containing Graphene and DOPA-Modified Graphene. PMID:39065398 | 2024 | this review only | not stated | not extracted yet |
| Wu 2023 Oxidized Dopamine Acrylamide Primer to Achieve Durable Resin-Dentin Bonding. PMID:37040295 | 2023 | this review only | not stated | not extracted yet |
| Xu 2022 A mussel glue-inspired monomer-etchant cocktail for improving dentine bonding DOI:10.1016/j.jdent.2021.103888 | 2022 | this review only | not stated | not extracted yet |
| Xu 2022 A mussel glue-inspired monomer-etchant cocktail for improving dentine bonding. PMID:34762990 | 2022 | this review only | not stated | not extracted yet |
| Yao 2024 High‐Performance Bioinspired Microspheres for Boosting Dental Adhesion DOI:10.1002/smll.202310251 | 2024 | this review only | not stated | not extracted yet |
| Yao 2024 High-Performance Bioinspired Microspheres for Boosting Dental Adhesion. PMID:38362704 | 2024 | this review only | not stated | not extracted yet |
| Zhang 2020 Enhancement performance of application mussel-biomimetic adhesive primer for dentin adhesives DOI:10.1039/c9ra10992g | 2020 | this review only | not stated | not extracted yet |
| Zhang 2020 Enhancement performance of application mussel-biomimetic adhesive primer for dentin adhesives. PMID:35496601 | 2020 | this review only | not stated | not extracted yet |
Review question
| Element | Planned (methods) | Found (results) |
|---|---|---|
| Population | Sound human or bovine teeth | Human molars and bovine incisors (n=13 studies; 12 in meta-analysis) |
| Intervention | Application of mussel-inspired compounds in dentin bonding | Monomeric (DMA, CLM, OX-DAA, L-DOPA; n=10) and polymeric (pDMA-MEA, PDA, MAP; n=3) mussel-inspired compounds |
| Comparison | Control group without mussel-inspired compounds | Water, ethanol, DMSO, no pretreatment, phosphoric acid/DMSO, positive controls (gold-standard self-etch adhesive; experimental adhesive) |
| Outcomes | Resin-dentin bond performance (microtensile and shear bond strength; secondary: protease inhibition, hydroxyproline release, collagen degradation) | Immediate bond strength (n=11); reduction of bond strength after aging (n=8); hydroxyproline release (n=4); in situ zymography and enzyme activity assays (descriptive) |
| Study designs | In vitro or ex vivo studies, English language | In vitro studies (n=13) |
| Timing | Not specified | Immediate and after aging (thermocycling 2,500 to 20,000 cycles; one study with collagenase challenge) |
AMSTAR 2 items
Two independent extractors rated each item. Their ratings are shown side by side because they have not been reconciled yet. The overall rating follows Shea et al. 2017: one critical flaw gives Low, more than one gives Critically Low.
| Item | Extractor A | Extractor B | Agree |
|---|---|---|---|
| 1Research question includes PICO components | Yes | Yes | yes |
| 2Protocol established before the reviewcritical | Partial Yes | Partial Yes | yes |
| 3Choice of study designs explained | No | No | yes |
| 4Comprehensive literature searchcritical | Partial Yes | Partial Yes | yes |
| 5Study selection in duplicate | Yes | Yes | yes |
| 6Data extraction in duplicate | Yes | Yes | yes |
| 7List of excluded studies with reasonscritical | No | No | yes |
| 8Included studies described in detail | Yes | Partial Yes | no |
| 9Risk of bias assessed satisfactorilycritical | RCT: N/A · NRSI: Partial Yes | NRSI: Partial Yes · RCT: N/A | no |
| 10Funding of included studies reported | No | No | yes |
| 11Appropriate meta-analysis methodscritical | RCT: N/A · NRSI: Yes | NRSI: Partial Yes · RCT: N/A | no |
| 12Impact of risk of bias on the pooled results | No | No | yes |
| 13Risk of bias accounted for in interpretationcritical | Yes | Yes | yes |
| 14Heterogeneity explained | Yes | Yes | yes |
| 15Publication bias investigatedcritical | Yes | Yes | yes |
| 16Conflicts of interest reported | Yes | Yes | yes |
| Overall | Low | Low | yes |