Non-Cochrane · intervention · PM31340425
Effect of Photodynamic Therapy on Microorganisms Responsible for Dental Caries: A Systematic Review and Meta-Analysis.
de Oliveira Analú Barros, Ferrisse Túlio Morandin, Marques Raquel Souza, et al. · International journal of molecular sciences · 2019 · PubMed · doi:10.3390/ijms20143585
Full textEvery question was extracted from the full text, figures and supplements by two independent LLM extractors, disagreements adjudicated. Rows cite where in the review the value comes from.
Questions
2
current version
GRADE rated
0
No studies
0
Included studies
65
linked below
No studies 0 · 0%Ungraded 2 · 100%Very low 0 · 0%Low 0 · 0%Moderate 0 · 0%High 0 · 0%
Ungraded means an effect was reported without a GRADE rating; it is not a gap. No studies means the review found nothing to answer the question. The six categories are a display order, not a score.
Review question
| Element | Planned (methods) | Found (results) |
|---|---|---|
| Population | Cariogenic microorganisms | Cariogenic microorganisms, most often Streptococcus mutans (82%); also multispecies biofilms, Lactobacillus and Candida (n=34) |
| Intervention | Photodynamic therapy (PDT) | PDT with various photosensitizers (methylene blue most common) and light sources (red LED 625 to 670 nm most common); pre-irradiation 1 to 30 min (n=34) |
| Comparison | Non-photodynamic therapy (no PDT applied) | Untreated negative control most common; some chlorhexidine or other comparators (n=34) |
| Outcomes | Microbial reduction | Microbial reduction in log CFU/mL; most studies achieved less than 3 log reduction (n=34) |
| Study designs | All study designs eligible (in vitro, in situ, in vivo, randomized clinical trial, case cohort, case control) using cariogenic biofilm models; English-language only | 30 in vitro studies and 4 randomized controlled trials (n=34) |
| Timing | Not specified | Not specified (pre-irradiation times 1 to 30 min reported) |
Search date: December 1989 to March 2019. Databases: PubMed; Embase; SCOPUS; Lilacs; Science Direct; Web of Science; Medline; SCIELO; Cochrane Library
Questions and results
Antimicrobial photodynamic therapy (PDT) vs No photodynamic therapy (untreated negative control)
Population: Cariogenic microorganisms in vitro (Streptococcus mutans and multispecies cariogenic biofilms)
| Outcome | Effect | Evidence | Status / GRADE |
|---|---|---|---|
Microbial reduction (viable count, log CFU/mL) In vitro studies In in vitro studies, photodynamic therapy significantly reduced cariogenic microbial counts versus untreated control (SMD -2.64, 95% CI -4.50 to -0.79; 2 studies; I2=0%); certainty was not graded. Source: Figure 2A; Results Section 2.4 Meta-Analysis; Methods Section 4.4 · same question in other reviews | SMD -2.64 [-4.50, -0.79] | 2 studies · 14 participants | Ungraded |
Antimicrobial photodynamic therapy (PDT) vs No photodynamic therapy (untreated negative control)
Population: Patients with dental caries (in vivo cariogenic microorganisms, including children with early childhood caries)
| Outcome | Effect | Evidence | Status / GRADE |
|---|---|---|---|
Microbial reduction (viable count, log CFU/mL) Randomized clinical trials In randomized clinical trials, photodynamic therapy significantly reduced cariogenic microbial counts versus untreated control (SMD -1.16, 95% CI -2.02 to -0.30; 2 trials), but heterogeneity was high (I2=70%) and considered too high to rely on; certainty was not graded. Source: Figure 2B; Results Section 2.4 Meta-Analysis; Methods Section 4.4 · same question in other reviews | SMD -1.16 [-2.02, -0.30] | 2 studies · 42 participants | Ungraded |
AMSTAR 2 appraisal
Two independent extractors rated each item. Their ratings are shown side by side because they have not been reconciled yet. The overall rating follows Shea et al. 2017: one critical flaw gives Low, more than one gives Critically Low.
| Item | Extractor A | Extractor B | Agree |
|---|---|---|---|
| 1Research question includes PICO components | Yes | Yes | yes |
| 2Protocol established before the reviewcritical | No | No | yes |
| 3Choice of study designs explained | No | No | yes |
| 4Comprehensive literature searchcritical | Partial Yes | Partial Yes | yes |
| 5Study selection in duplicate | Yes | No | no |
| 6Data extraction in duplicate | No | No | yes |
| 7List of excluded studies with reasonscritical | No | No | yes |
| 8Included studies described in detail | Partial Yes | Yes | no |
| 9Risk of bias assessed satisfactorilycritical | RCT: No · NRSI: No | NRSI: N/A · RCT: No | no |
| 10Funding of included studies reported | No | No | yes |
| 11Appropriate meta-analysis methodscritical | RCT: Yes · NRSI: Yes | NRSI: N/A · RCT: Yes | no |
| 12Impact of risk of bias on the pooled results | No | No | yes |
| 13Risk of bias accounted for in interpretationcritical | No | No | yes |
| 14Heterogeneity explained | Yes | Yes | yes |
| 15Publication bias investigatedcritical | No | No | yes |
| 16Conflicts of interest reported | Yes | Yes | yes |
| Overall | Critically Low | Critically Low | yes |
Included primary studies 65
Open as a trial list| Study | Also included by | Outcomes it informs here | Trial PICO | Results |
|---|---|---|---|---|
| Alexandrino 2019 Rose Bengal incorporated to α-cyclodextrin microparticles for photodynamic therapy against the cariogenic microorganism Streptococcus… PMID:30468898 | this review only | not stated | not extracted yet | not extracted yet |
| Alexandrino, 2019 Rose Bengal incorporated to α-cyclodextrin microparticles for photodynamic therapy against the cariogenic microorganism Streptococcus mutans DOI:10.1016/j.pdpdt.2018.11.016 | this review only | not stated | not extracted yet | not extracted yet |
| Araujo 2014 Photodynamic antimicrobial therapy of curcumin in biofilms and carious dentine. PMID:23793414 | this review only | not stated | not extracted yet | not extracted yet |
| Araújo, 2014 Photodynamic antimicrobial therapy of curcumin in biofilms and carious dentine DOI:10.1007/s10103-013-1369-3 | this review only | not stated | not extracted yet | not extracted yet |
| Azizi 2018 Comparison of the antimicrobial efficacy of photodynamic therapy with two mediators against Lactobacillus acidophilus in vitro. PMID:29409897 | this review only | not stated | not extracted yet | not extracted yet |
| Azizi, 2018 Comparison of the antimicrobial efficacy of photodynamic therapy with two mediators against Lactobacillus acidophilus in vitro DOI:10.1016/j.pdpdt.2018.01.014 | this review only | not stated | not extracted yet | not extracted yet |
| Beytollahi 2017 The efficacy of photodynamic and photothermal therapy on biofilm formation of Streptococcus mutans: An in vitro study. PMID:27769914 | this review only | not stated | not extracted yet | not extracted yet |
| Beytollahi, 2017 The efficacy of photodynamic and photothermal therapy on biofilm formation of Streptococcus mutans: An in vitro study DOI:10.1016/j.pdpdt.2016.10.006 | this review only | not stated | not extracted yet | not extracted yet |
| Cusicanqui Mendez 2018 Curcumin-mediated antimicrobial photodynamic therapy reduces the viability and vitality of infected dentin caries microcosms. PMID:30240927 | this review only | not stated | not extracted yet | not extracted yet |
| Cusicanqui Méndez, 2018 Curcumin-mediated antimicrobial photodynamic therapy reduces the viability and vitality of infected dentin caries microcosms DOI:10.1016/j.pdpdt.2018.09.007 | this review only | not stated | not extracted yet | not extracted yet |
| Darmani 2018 Comparison of the Photosensitivity of Biofilms of Different Genera of Cariogenic Bacteria in Tooth Slices. PMID:30550231 | this review only | not stated | not extracted yet | not extracted yet |
| de Oliveira 2018 The in vitro effect of Antimicrobial Photodynamic Therapy on dental microcosm biofilms from partially erupted permanent molars: A pilot… PMID:29233757 | this review only | not stated | not extracted yet | not extracted yet |
| de Oliveira, 2018 The in vitro effect of Antimicrobial Photodynamic Therapy on dental microcosm biofilms from partially erupted permanent molars: A pilot… DOI:10.1016/j.pdpdt.2017.12.005 | this review only | not stated | not extracted yet | not extracted yet |
| Diniz 2015 Antimicrobial photodynamic therapy: a promise candidate for caries lesions treatment. PMID:26002012 | this review only | not stated | not extracted yet | not extracted yet |
| Diniz, 2015 Antimicrobial photodynamic therapy: A promise candidate for caries lesions treatment DOI:10.1016/j.pdpdt.2015.04.006 | this review only | not stated | not extracted yet | not extracted yet |
| Esper 2019 Photodynamic inactivation of planktonic cultures and Streptococcus mutans biofilms for prevention of white spot lesions during orthodontic… PMID:30712696 | this review only | not stated | not extracted yet | not extracted yet |
| Esper, 2019 Photodynamic inactivation of planktonic cultures and Streptococcus mutans biofilms for prevention of white spot lesions during orthodontic… DOI:10.1016/j.ajodo.2018.03.027 | this review only | not stated | not extracted yet | not extracted yet |
| Esteban Florez 2018 Bioluminescence Analysis of Antibacterial Photodynamic Therapy Using Methylene Blue Mediated by Low-Intensity Level Laser Against… PMID:29315011 | this review only | not stated | not extracted yet | not extracted yet |
| Esteban Florez, 2018 Bioluminescence Analysis of Antibacterial Photodynamic Therapy Using Methylene Blue Mediated by Low-Intensity Level Laser Against… DOI:10.1089/pho.2017.4326 | this review only | not stated | not extracted yet | not extracted yet |
| Fekrazad 2013 Photoelimination of Streptococcus mutans with two methods of photodynamic and photothermal therapy. PMID:24284120 | this review only | not stated | not extracted yet | not extracted yet |
| Fekrazad 2017 Effect of antimicrobial photodynamic therapy on the counts of salivary Streptococcus mutans in children with severe early childhood caries. PMID:28359938 | this review only | Microbial reduction (viable count, log CFU/mL) | not extracted yet | not extracted yet |
| Fekrazad, 2013 Photoelimination of Streptococcus mutans with two methods of photodynamic and photothermal therapy DOI:10.1016/j.pdpdt.2013.07.002 | this review only | not stated | not extracted yet | not extracted yet |
| Fekrazad, 2017 Effect of antimicrobial photodynamic therapy on the counts of salivary Streptococcus mutans in children with severe early childhood caries DOI:10.1016/j.pdpdt.2017.03.007 | this review only | Microbial reduction (viable count, log CFU/mL) | not extracted yet | not extracted yet |
| Fumes 2018 Influence of pre-irradiation time employed in antimicrobial photodynamic therapy with diode laser. PMID:28963589 | this review only | Microbial reduction (viable count, log CFU/mL) | not extracted yet | not extracted yet |
| Fumes, 2018 Influence of pre-irradiation time employed in antimicrobial photodynamic therapy with diode laser DOI:10.1007/s10103-017-2336-1 | this review only | Microbial reduction (viable count, log CFU/mL) | not extracted yet | not extracted yet |
| Garcia 2018 Photodynamic therapy mediated by chlorin-type photosensitizers against Streptococcus mutans biofilms. PMID:30157462 | this review only | not stated | not extracted yet | not extracted yet |
| Garcia, 2018 Photodynamic therapy mediated by chlorin-type photosensitizers against Streptococcus mutans biofilms DOI:10.1016/j.pdpdt.2018.08.012 | this review only | not stated | not extracted yet | not extracted yet |
| Gholibegloo 2018 Carnosine-graphene oxide conjugates decorated with hydroxyapatite as promising nanocarrier for ICG loading with enhanced antibacterial… PMID:29482032 | this review only | not stated | not extracted yet | not extracted yet |
| Gholibegloo, 2018 Carnosine-graphene oxide conjugates decorated with hydroxyapatite as promising nanocarrier for ICG loading with enhanced antibacterial… DOI:10.1016/j.jphotobiol.2018.02.004 | this review only | not stated | not extracted yet | not extracted yet |
| Gomez 2018 Efficacy of photodynamic therapy vs ultrasonic scaler for preventing gingival inflammation and white spot lesions during orthodontic… PMID:30399455 | this review only | not stated | not extracted yet | not extracted yet |
| Gómez, 2018 Efficacy of photodynamic therapy vs ultrasonic scaler for preventing gingival inflammation and white spot lesions during orthodontic… DOI:10.1016/j.pdpdt.2018.11.001 | this review only | not stated | not extracted yet | not extracted yet |
| Lamarque 2019 Could chlorhexidine be an adequate positive control for antimicrobial photodynamic therapy in- in vitro studies? PMID:30399454 | this review only | Microbial reduction (viable count, log CFU/mL) | not extracted yet | not extracted yet |
| Lamarque, 2019 Could chlorhexidine be an adequate positive control for antimicrobial photodynamic therapy in- in vitro studies? DOI:10.1016/j.pdpdt.2018.11.004 | this review only | Microbial reduction (viable count, log CFU/mL) | not extracted yet | not extracted yet |
| Lara Alves 2019 Influence of antimicrobial photodynamic therapy in carious lesion. Randomized split-mouth clinical trial in primary molars. PMID:30807834 | this review only | Microbial reduction (viable count, log CFU/mL) | not extracted yet | not extracted yet |
| Lara Alves, 2019 Influence of antimicrobial photodynamic therapy in carious lesion. Randomized split-mouth clinical trial in primary molars DOI:10.1016/j.pdpdt.2019.02.018 | this review only | Microbial reduction (viable count, log CFU/mL) | not extracted yet | not extracted yet |
| Leal 2017 Antimicrobial photodynamic therapy on Streptococcus mutans is altered by glucose in the presence of methylene blue and red LED. PMID:28414082 | this review only | not stated | not extracted yet | not extracted yet |
| Leal, 2017 Antimicrobial photodynamic therapy on Streptococcus mutans is altered by glucose in the presence of methylene blue and red LED DOI:10.1016/j.pdpdt.2017.04.004 | this review only | not stated | not extracted yet | not extracted yet |
| Lee 2017 Antibacterial photodynamic therapy with curcumin and Curcuma xanthorrhiza extract against Streptococcus mutans. PMID:28890211 | this review only | not stated | not extracted yet | not extracted yet |
| Lee, 2017 Antibacterial photodynamic therapy with curcumin and Curcuma xanthorrhiza extract against Streptococcus mutans DOI:10.1016/j.pdpdt.2017.09.003 | this review only | not stated | not extracted yet | not extracted yet |
| Luthi 2009 Hypericin- and mTHPC-mediated photodynamic therapy for the treatment of cariogenic bacteria DOI:10.1016/j.mla.2009.07.004 | this review only | not stated | not extracted yet | not extracted yet |
| Mang 2012 Photodynamic therapy as an alternative treatment for disinfection of bacteria in oral biofilms. PMID:22847720 | this review only | not stated | not extracted yet | not extracted yet |
| Mang, 2012 Photodynamic therapy as an alternative treatment for disinfection of bacteria in oral biofilms DOI:10.1002/lsm.22050 | this review only | not stated | not extracted yet | not extracted yet |
| Manoil 2014 Flow cytometric assessment of Streptococcus mutans viability after exposure to blue light-activated curcumin. PMID:24973577 | this review only | not stated | not extracted yet | not extracted yet |
| Manoil, 2014 Flow cytometric assessment of Streptococcus mutans viability after exposure to blue light-activated curcumin DOI:10.1016/j.pdpdt.2014.06.003 | this review only | not stated | not extracted yet | not extracted yet |
| Melo 2015 Photodynamic antimicrobial chemotherapy and ultraconservative caries removal linked for management of deep caries lesions. PMID:26431977 | this review only | not stated | not extracted yet | not extracted yet |
| Melo, 2015 Photodynamic antimicrobial chemotherapy and ultraconservative caries removal linked for management of deep caries lesions DOI:10.1016/j.pdpdt.2015.09.005 | this review only | not stated | not extracted yet | not extracted yet |
| Muller 2007 Efficacy of gasiform ozone and photodynamic therapy on a multispecies oral biofilm in vitro. PMID:17305720 | this review only | not stated | not extracted yet | not extracted yet |
| Müller, 2007 Efficacy of gasiform ozone and photodynamic therapy on a multispecies oral biofilm <i>in vitro</i> DOI:10.1111/j.1600-0722.2007.00418.x | this review only | not stated | not extracted yet | not extracted yet |
| Nemezio 2017 Effect of methylene blue-induced photodynamic therapy on a Streptococcus mutans biofilm model. PMID:29101088 | this review only | not stated | not extracted yet | not extracted yet |
| Nemezio, 2017 Effect of methylene blue-induced photodynamic therapy on a Streptococcus mutans biofilm model DOI:10.1016/j.pdpdt.2017.10.025 | this review only | not stated | not extracted yet | not extracted yet |
| Perez-Laguna 2017 Comparative effect of photodynamic therapy on separated or mixed cultures of Streptococcus mutans and Streptococcus sanguinis. PMID:28546111 | this review only | not stated | not extracted yet | not extracted yet |
| Pérez-Laguna, 2017 Comparative effect of photodynamic therapy on separated or mixed cultures of Streptococcus mutans and Streptococcus sanguinis DOI:10.1016/j.pdpdt.2017.05.017 | this review only | not stated | not extracted yet | not extracted yet |
| Pourhajibagher 2019 Physico-mechanical and antimicrobial properties of an orthodontic adhesive containing cationic curcumin doped zinc oxide nanoparticles… PMID:30611862 | this review only | not stated | not extracted yet | not extracted yet |
| Pourhajibagher, 2019 Physico-mechanical and antimicrobial properties of an orthodontic adhesive containing cationic curcumin doped zinc oxide nanoparticles… DOI:10.1016/j.pdpdt.2019.01.002 | this review only | not stated | not extracted yet | not extracted yet |
| Rolim 2012 The antimicrobial activity of photodynamic therapy against Streptococcus mutans using different photosensitizers. PMID:22070899 | this review only | not stated | not extracted yet | not extracted yet |
| Rolim, 2012 The antimicrobial activity of photodynamic therapy against Streptococcus mutans using different photosensitizers DOI:10.1016/j.jphotobiol.2011.10.001 | this review only | not stated | not extracted yet | not extracted yet |
| Soria-Lozano 2015 In vitro effect photodynamic therapy with differents photosensitizers on cariogenic microorganisms. PMID:26410025 | this review only | not stated | not extracted yet | not extracted yet |
| Spinei 2013 PMID:21232013 | this review only | not stated | not extracted yet | not extracted yet |
| Spinei, 2013 matched by author and year (probable) | this review only | not stated | not extracted yet | not extracted yet |
| Tokubo 2018 Antimicrobial effect of photodynamic therapy using erythrosine/methylene blue combination on Streptococcus mutans biofilm. PMID:29763739 | this review only | not stated | not extracted yet | not extracted yet |
| Tokubo, 2018 Antimicrobial effect of photodynamic therapy using erythrosine/methylene blue combination on Streptococcus mutans biofilm DOI:10.1016/j.pdpdt.2018.05.004 | this review only | not stated | not extracted yet | not extracted yet |
| Trigo-Gutierrez 2018 Effect of Chloroaluminium phthalocyanine in cationic nanoemulsion on photoinactivation of multispecies biofilm. PMID:30308310 | this review only | not stated | not extracted yet | not extracted yet |
| Trigo-Gutierrez, 2018 Effect of Chloroaluminium phthalocyanine in cationic nanoemulsion on photoinactivation of multispecies biofilm DOI:10.1016/j.pdpdt.2018.10.005 | this review only | not stated | not extracted yet | not extracted yet |
| Zanin 2006 Photosensitization of in vitro biofilms by toluidine blue O combined with a light-emitting diode. PMID:16460343 | this review only | not stated | not extracted yet | not extracted yet |
| Zanin, 2006 Photosensitization of <i>in vitro</i> biofilms by toluidine blue O combined with a light‐emitting diode DOI:10.1111/j.1600-0722.2006.00263.x | this review only | not stated | not extracted yet | not extracted yet |